OCT/LCT monitoring of drug action on the structure of the human cornea in vivo

Elina A. Genina (Login required)
N.G. Chernyshevsky Saratov State University, Russia,
Tomsk State University, Russia

Alexey N. Bashkatov
N.G. Chernyshevsky Saratov State University, Russia,
Tomsk State University, Russia

Ivan D. Kamenskikh
V.I. Razumovsky Saratov State Medical University, Russia

Igor O. Kolbenev
V.I. Razumovsky Saratov State Medical University, Russia

Tatiana G. Kamenskikh
V.I. Razumovsky Saratov State Medical University, Russia

Valery V. Tuchin
N.G. Chernyshevsky Saratov State University, Russia,
Tomsk State University, Russia,
Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russia


Paper #1989 received 2014.12.17; revised manuscript received 2015.01.21; accepted for publication 2015.01.21; published online 2015.03.28.

DOI: 10.18287/jbpe-2015-1-1-77

Abstract

The effect of anti-glaucoma medicinal prepaprations Timolol-AKOS and Cosopt on the structure components of human eye cornea are studied. The eye drops Timolol-AKOS 0.5% and Cosopt were used as the object of study. 10 voluntary patients of the Eye Disease Clinic aged from 70 to 75 suffering from glaucoma took part in the studies. The study of cornea in vivo was carried out using the methods of laser scanning confocal tomography (LCT) and optical coherence tomography (OCT) before the application of drugs and at different time moments after the application. The eye cornea thickness values measured using the OCT at the initial moment of time and at different times after the application of the medicinal preparations are presented. From the LCT data the density and the mean diameter of the cornea epithelial cells was calculated. As a result of the complex studies carried out, it was shown that the Timolol-AKOS medication causes the swelling of the cornea within the limits of 1-5%. Under the use of Cosopt medication the cornea dehydration was observed.

Keywords

Glaucoma; cornea; optical coherence tomography; confocal microscopy

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References


1. Y. L. Kim, J. T. Walsh Jr., T. K. Goldstick, and M. R. Glucksberg, “Variation of corneal refractive index with hydration,” Phys. Med. Biol. 49, 859-868 (2004). Crossref

2. K. V. Larin, and M. G. Ghosn, “Optical coherent tomography measurements of the diffusion rate of water and drugs in an isolated and whole cornea,” Quantum Electronics 36(12), 1083-1088 (2006). Crossref

3. Y. Wu, D. Clarke, A. Mathew, I. Nicoud, and X. Li, “Noninvasive optical coherence tomography monitoring of structure and hydration changes of human corneas in different preservation media,” J. Biomed. Opt. 16(2), 026015 (2011). Crossref

4. M. G. Ghosn, V. V. Tuchin, and K. V. Larin, “Nondestructive quantification of analyte diffusion in cornea and sclera using optical coherence tomography,” Invest. Ophthalmol. & Vis. Sci. 48(6), 2726-2733 (2007). Crossref

5. M. Böhnke, and B. R. Masters, “Confocal microscopy of the cornea,” Progress in Retinal and Eye Research 18(5), 553-628 (1999). Crossref

6. S. E. Avetisov, G. B. Egorova, A. A. Fedorov, and N. V. Bobrovskikh, “Confocal microscopy of cornea. Communication 1. The features of normal morphology pattern,” Vestnik oftalmologii 3, 3-5 (2008) (in Russian).

7. I. G. Smetankin, and D. I. Agarkova, “Confocal microscopy and optical coherence tomography in the assessment of anatomy and functional condition of a cornea wound (in vivo) after the phacoemulsification of cataract,” Sovremennye tekhnologii v medistine 3, 89-92 (2012) (in Russian).

8. Y. Zhou, K. K. H. Chan, T. Lai, and S. Tang, “Characterizing refractive index and thickness of biological tissues using combined multiphoton microscopy and optical coherence tomography,” Biomedical Optics Express 4(1), 38-50 (2013). Crossref

9. D. E. Freund, R. L. McCally, and R. A. Farrell, “Effects of fibril orientations on light scattering in the cornea,” J. Opt. Soc. Am. A. 3, 1970-1982 (1986). Crossref

10. R. A. Farrell, D. E. Freund, and R. L. McCally, “Research on corneal structure,” Johns Hopkins Appl. Physics Lab. Techn. Digest. 11(1,2) 191-199 (1990).

11. A. S. Orlova, A. N. Bashkatov, E. A. Genina, I. O. Kolbenev, I. D. Kamenskikh, T. G. Kamenskikh, and V. V. Tuchin, “Influence of 40%-glucose solution on a human corneal structure,” Izvestiya Saratov. Universiteta. New ser. Ser. Physics 14(1), 11-19 (2014).






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