Software development for estimation of optical clearing agent’s diffusion coefficients in biological tissues

Pedro Peixoto
Physics Department – Polytechnic Institute of Porto, School of Engineering, Portugal

Luís Oliveira (Login required)
Physics Department – Polytechnic Institute of Porto, School of Engineering, Portugal,
FEUP – University of Porto, Portugal,
CIETI – Centre of Innovation in Engineering and Industrial Technology, ISEP, Porto, Portugal

Maria Inês Carvalho
FEUP – University of Porto, Portugal
DEEC/FEUP and INESC TEC, University of Porto, Portugal

Elisabete Nogueira
Physics Department – Polytechnic Institute of Porto, School of Engineering, Portugal,
CIETI – Centre of Innovation in Engineering and Industrial Technology, ISEP, Porto, Portugal

Valery V. Tuchin
Research-Educational Institute of Optics and Biophotonics, Saratov National Research State University, Russia
Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Saratov, Russia
Interdisciplinary Laboratory of Biophotonics, Tomsk National Research State University, Russia


Paper #2815 received 2015.12.17; revised manuscript received 2015.12.30; accepted for publication 2015.12.31; published online 2016.02.05.

DOI: 10.18287/JBPE-2015-1-4-255

Abstract

The study of chemical diffusion in biological tissues is a research field of high importance and with application in many clinical, research and industrial areas. The evaluation of diffusion and viscosity properties of chemicals in tissues is necessary to characterize treatments or inclusion of preservatives in tissues or organs for low temperature conservation. Recently, we have demonstrated experimentally that the diffusion properties and dynamic viscosity of sugars and alcohols can be evaluated from optical measurements. Our studies were performed in skeletal muscle, but our results have revealed that the same methodology can be used with other tissues and different chemicals. Considering the significant number of studies that can be made with this method, it becomes necessary to turn data processing and calculation easier. With this objective, we have developed a software application that integrates all processing and calculations, turning the researcher work easier and faster. Using the same experimental data that previously was used to estimate the diffusion and viscosity of glucose in skeletal muscle, we have repeated the calculations with the new application. Comparing between the results obtained with the new application and with previous independent routines we have demonstrated great similarity and consequently validated the application. This new tool is now available to be used in similar research to obtain the diffusion properties of other chemicals in different tissues or organs.

Keywords

Collimated optical transmittance; Chemical diffusion in tissues; Glucose; Optical clearing; Refractive index matching; Thickness variation; Viscosity; Software application.

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