Software development for estimation of optical clearing agent’s diffusion coefficients in biological tissues
Paper #2815 received 2015.12.17; revised manuscript received 2015.12.30; accepted for publication 2015.12.31; published online 2016.02.05.
The study of chemical diffusion in biological tissues is a research field of high importance and with application in many clinical, research and industrial areas. The evaluation of diffusion and viscosity properties of chemicals in tissues is necessary to characterize treatments or inclusion of preservatives in tissues or organs for low temperature conservation. Recently, we have demonstrated experimentally that the diffusion properties and dynamic viscosity of sugars and alcohols can be evaluated from optical measurements. Our studies were performed in skeletal muscle, but our results have revealed that the same methodology can be used with other tissues and different chemicals. Considering the significant number of studies that can be made with this method, it becomes necessary to turn data processing and calculation easier. With this objective, we have developed a software application that integrates all processing and calculations, turning the researcher work easier and faster. Using the same experimental data that previously was used to estimate the diffusion and viscosity of glucose in skeletal muscle, we have repeated the calculations with the new application. Comparing between the results obtained with the new application and with previous independent routines we have demonstrated great similarity and consequently validated the application. This new tool is now available to be used in similar research to obtain the diffusion properties of other chemicals in different tissues or organs.
1. F. E. Dunlap, O. H. Mills, M. R. Tuley, M. D. Baker, and R. T. Plott, “Adapalene 0.1% gel for the treatment of acne vulgaris: its superiority compared to tretinoin 0.025% cream in skin tolerance and patient preference,” British Journal of Dermatology 139(52), 17–22 (1998). Crossref
2. E. A. Genina, A. N. Bashkatov, Y. P. Sinichkin, I. Y. Yanina, and V. V. Tuchin, “Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy,” J. of Biomedical Photonics and Engineering 1(1), 22–58 (2015). Crossref
3. L. M. Alvarez, Compendium of Organ & Tissue Banking Concepts (2015).
4. R. Bertram, and M. Pernarowski, “Glucose diffusion in pancreatic islets of Langerhans,” Biophys. J. 74(4), 1722–1731 (1998).
5. A. C. Ribeiro, O. Ortona, S. M. N. Simões, C. I. A. V. Santos, P. M. R. A. Prazeres, A. J. M. Valente, V. M. M. Lobo, and H. D. Burrows, “Binary mutual diffusion coefficients of aqueous solutions of sucrose, lactose, glucose and fructose in the temperature range from (298.15 to 328.15) K,” J. Chem. Eng. Data 51(5), 1836–1840 (2006).
6. M. G. Ghosn, E. F. Carbajal, N. A. Befrui, V. V. Tuchin, and K. V. Larin, “Concentration effect on the diffusion of glucose in ocular tissues,” Opt. Laser Eng. 46(12), 911–914, (2008).
7. L. M. Oliveira, M. I. Carvalho, E. M. Nogueira, and V. V. Tuchin, “The characteristic time of glucose diffusion measured for muscle tissue at optical clearing,” Laser Physics 23(7), 075606 (2013). Crossref
8. A. N. Bashkatov, E. A. Genina, and V. V. Tuchin, “Measurement of glucose diffusion coefficients in human tissues,” Chap. 19 in Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues, V. V. Tuchin, (ed.), Taylor & Francis Group LLC, CRC Press, London, 587–621 (2009).
9. M. Kreft, M. Lukši?, T. M. Zorec, M. Prebil, and R. Zorec, “Diffusion of D-glucose measured in the cytosol of a single astrocyte,” Cell. Mol. Life Sci. 70(8), 1483–1492 (2013).
10. L. Oliveira et al., “Optical clearing mechanisms characterization in muscle”, to be published.
11. V. V. Tuchin, I. L. Maksimova, D. A. Zimnyakov, I. L. Kon, A. H. Mavlyutov, and A. A. Mishin, “Light propagation in tissues with controlled optical properties,” J. Biomed. Opt. 2(4), 401–407 (1997).
12. V. V. Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis, 3rd Ed., Bellingham: SPIE Press (2015).
13. V. V. Tuchin, Optical Clearing of Tissues and Blood, Bellingham, SPIE Press (2006).
14. E. A. Genina, A. N. Bashkatov, and V. V. Tuchin, “Tissue optical immersion clearing,” Expert Rev. Med. Devices 7(6), 825–842 (2010). Crossref
15. K. Becker, N. Jährling, S. Saghafi, R. Weiler, and H-U. Dodt, “Chemical clearing and dehydration of GFP expressing mouse brains,” PLoS One 7(3), e33916 (2012).
16. K. Becker, N. Jährling, S. Saghafi, and H-U. Dodt, “Dehydration and clearing of whole mouse brains and dissected hippocampi for ultramicroscopy,” Cold Spring Harbour Protocol 2013(7), 683-684 (2011).
17. D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, “Recent progress in tissue optical clearing,” Laser Photonics Rev. 7(5), 732-757 (2013). Crossref
18. L. M. Oliveira, M. I. Carvalho, E. M. Nogueira, and V. V. Tuchin, “Diffusion characteristics of ethylene glycol in skeletal muscle,” J. Biomed. Opt. 20(5), 051019 (2015).
19. V. V. Larin, M. G. Ghosn, A. N. Bashkatov, E. A. Genina, N. A. Trunina, and V. V. Tuchin, “Optical clearing for OCT image enhancement and in-depth monitoring of molecular diffusion,” IEEE J. Select. Tops. Quant. Electr. 18(3), 1244-1259 (2012).
20. J. Wang, Y. Zhang, P. Li, Q. Luo, and D. Zhu, “Review: Tissue optical clearing window for blood flow monitoring,” IEEE J. Select. Tops. Quant. Electr. 20(2), 92-103 (2014).
21. O. Nadiarnykh, and P. J. Campagnola, “SHG and optical clearing,” in Second Harmonic Generation Imaging, F. S. Pavone, and P. J. Campagnola (eds.), CRC Press, Taylor & Francis Group, Boca Raton, London, NY, 169?189 (2014).
22. E. A. Genina, A. N. Bashkatov, and V. V. Tuchin, “Glucose-induced optical clearing effects in tissues and blood,” Chap. 21 in Handbook of Optical Sensing of Glucose in Biological Fluids and Tissues, V. V. Tuchin (ed.), Taylor & Francis Group LLC, CRC Press, London (2009).
23. A. Kotyk, and K. Janacek, Membrane Transport: An interdisciplinary Approach, Plenum Press, New York (1977). ISBN: 978-1-4684-3333-3.
24. H. Suhaimi, S. Wang. T. Thornton, and D. B. Das, “On glucose diffusivity of tissue engineering membranes and scaffolds,” Chemical Engineering Science 126, 244–256 (2015). Crossref
25. J. M. Christensen, M. C. Chuong, H. Le, L. Pham, and E. Bendas, “Hydrocortisone diffusion through synthetic membrane, mouse skin and epidermTM cultured skin,” Archives of Drug Information 4(1), 10–21 (2011). Crossref
26. P. N. Skandamis, and G.-J. E. Nychas, “Preservation of fresh meat with active and modified atmosphere packaging conditions,” Int. J. of Food Microbiology 79(1-2), 35–45 (2002). Crossref
27. G. H. Zhou, X. L. Xu, and Y. Liu, “Preservation technologies for fresh meat – A review,” Meat Science 86(1), 119–128 (2010).
28. D. Tiroutchelvame, V. Sivakumar, and J. P. Maran, “Optimization of mass transfer parameters during osmotic dehydration of momordica charantia slices,” J. of Food Processing and Preservation, (2015).
29. A. Bouchoux, H. Roux-de Balmann, and F. Lutin, “Nanofiltration of glucose and sodium lactate solutions: Variations of retention between single- and mixed-solute solutions,” J. of Membrane Science 258(1-2), 123–132 (2005).
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