The use of laser dynamical speckle interferometry in the study of cellular processes

A. P. Vladimirov
Ural Federal University named after the first President of Russia Boris N. Eltsin, Ekaterinburg, Russia
Ekaterinburg Research Institute of Virus Infections, Russia
Institute of Engineering Science, Urals Branch, Russian Academy of Sciences, Ekaterinburg, Russia

I. A. Novoselova (Login required)
Ural Federal University named after the first President of Russia Boris N. Eltsin, Ekaterinburg, Russia

Y. A. Mikhailova
Ural Federal University named after the first President of Russia Boris N. Eltsin, Ekaterinburg, Russia
Ekaterinburg Research Institute of Virus Infections, Russia

A. A. Bakharev
Ekaterinburg Research Institute of Virus Infections, Russia

D. I. Yakin
Ural Federal University named after the first President of Russia Boris N. Eltsin, Ekaterinburg, Russia


Paper #2952 received 2016.01.31; revised manuscript received 2016.02.12; accepted for publication 2016.03.03; published online 2016.03.30.

DOI: 10.18287/JBPE16.02.010302

Abstract

A speckle interferometry setup is mounted, tested and approved that allows the analysis of intracellular processes with large optical magnification. For different part of the cell, the dependences of speckle image correlation coefficients upon time are found. It is shown that the method allows the detection of differences in the process that occur in the nutrient solution outside the cell and inside the cell, as well as those occurring in different parts of the cell.

Keywords

laser; speckle; speckle interferometry; cell metabolism; correlation

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References


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