Long-term fluorescence of erythrosine in alive mammalian cells

Valeriya S. Maryakhina orcid (Login required)
Orenburg State University, Russia


Paper #3078 received 2016.07.18 revised manuscript received 2016.09.22; accepted for publication 2016.09.29; published online 2016.09.30.

DOI: 10.18287/JBPE16.02.030303

Abstract

Investigation of triplet states of probes molecules located in cells and tissues is used for research of pathological processes included cancer development due to high sensitivity of triplet states lifetime to surround medium. In the work the long-term fluorescence of erythrosine located in alive mammalian cells has been analyzed by mathematical simulation. The model considers all basic processes of relaxation of probe triplet states in biological media. It is obtained that signal intensity and its form depends on oxygen concentration and viscosity of cells cytoplasm in which the dye diffuses. Interaction of erythrosine with the variety of extinguishers (included immobilization process into peptides molecules) does not impact on signal of the delayed fluorescence. Their concentration impact on cell viscosity mean only. The most important extinguisher is molecular oxygen impacting on signal. The correlation between theoretical and experimental results let to consider that the model is adequate. 

Keywords

long-term fluorescence; erythrosine; singlet oxygen; mammalian cells; fluorescent diagnostics.

Full Text:

PDF

References


1. M. Scholz, A.-L. Biehl, R. Dědic, and J. Hála, “The singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopy approach,” Photochem. Photobiol. Sci. 14(4), 700–713 (2015).

2. F. A. Harms, W. M. de Boon, G. M. Balestra, S. I. Bodmer, T. Johannes, R. J. Stolker, and E. G. Mik, “Oxygen-dependent delayed fluorescence measured in skin after topical application of 5-aminolevulinic acid,” J. Biophotonics 4(10), 731–739 (2011). Crossref

3. V. V. Bryukhanov, G. A. Ketsle, V. C. Laurinas, L. V. Levshin, and Z. M. Muldakhmetov, “Singlet-triplet annihilation of singlet oxygen and triplet xanthene dye molecules in liquid solutions,” Journal of Applied Spectroscopy 46(4), 372–377 (1987). Crossref

4. E. A. Ermilov, O. L. Markovskii, and I. M. Gulis, “Inductive-resonant triplet-triplet annihilation in solid solutions of erythrosine,” Journal of applied spectroscopy 64(5), 642-645 (1997). Crossref

5. F. Kazmi, L. J. Haupt, J. R. Horkman, B. D. Smith, D. B. Buckley, E. A. Wachter, and J. M. Singer,. “In vitro inhibition of human liver cytochrome P450 (CYP) and UDP-glucuronosyltransferase (UGT) enzymes by rose bengal: system-dependent effects on inhibitory potential,” Xenobiotica 44(7), 606-614 (2014). Crossref

6. J. Lee, I. Kwon, S. S. Jang, and A. E. Cho, “Investigation of the effect of erythrosine B on amyloid beta peptide using molecular modeling,” Journal of molecular modeling 22(4), 1-9 (2016). Crossref

7. S. N. Letuta, and V. S. Maryakhina, “The delayed fluorescence kinetics as a method of biological tissue diagnostics”, Proc. SPIE 7999, 799907 (2011).

8. S. N. Letuta, V. S. Maryakhina, and R. R. Rakhmatullin, “Optical diagnostics of biological tissue cells during their cultivation in polymers,” Quantum electronics 41(4), 314-317 (2011).

9. K. Rohatgi Mukherjee, and A. K. Mukhopadhyay, ”Photophysical processes in halofluorescein dyes,” Indian Journal of Pure & Applied Physics 14(6), 481-484 (1976).

10. I. M. Vlasova, and A. M. Saletsky, “Fluorescent characteristics of probes of the fluorescein family in human serum albumin solutions,” Moscow Univ. Phys. Bull. 64, 412–416 (2009).

11. V. V. Bryukhanov, G. A. Ketsle, V. C. Laurinas, and L. V. Levshin, ”Oxygen induction of delayed fluorescence of fluorescenic dye liquid solutions,” Optika i Spektroskopiya 60(1), 205-207 (1986).

12. A. M. Mastro, M. A. Babich, W. D. Taylor, and A. D. Keith, “Diffusion of a small molecule in the cytoplasm of mammalian cells,” Proc. Natl. Acad. Sci. 81(11), 3414–3418 (1984).

13. A. G. Melnikov, A. B. Pravdin, V. I. Kochubey, A. V. Kuptsova, and G. V. Melnikov, “Interglobular diffusion of an energy donor in triplet-triplet energy transfer in proteins,” J. Spectrosc. 2013, 1-7 (2013).

14. A. A. Krasnovsky, “Luminescence and photochemical studies of singlet oxygen photonics,” Journal of Photochemistry and Photobiology A: Chemistry 196(2-3), 210–218 (2008). Crossref

15. S. Suresh, “Biomechanics and biophysics of cancer cells,” Acta Biomaterialia 3(4), 413–438 (2007). Crossref

16. R. B. Gorenkov, V. N. Karpov, D. A. Rogatkin, and V. I. Shumskiy, “Chronic hypoxia state as one of the factors of increased fluorescence of endogenous porphyrins in alive biological tissues,” Biophysics 52(4), 711-714 (2007).






© 2014-2017 Samara National Research University. All Rights Reserved.
Public Media Certificate (RUS). 12+