Photosensitizing properties of supramolecular systems based on chlorin e6
Paper #3122 received 2016.11.20; accepted for publication 2016.12.29; published online 2016.12.31.
The novel supramolecular systems based on chlorine e6 (Ce6) are presented, and their optical absorption and fluorescence have been investigated. The influence of different excipients as poly-N-vinylpyrrolidone (PVP), polyethyleneglycol (PEG), bovine serum albumin (BSA), chitosan, Triton X-100 (TX-100) on spectral characteristics of these systems has been studied. The obtained spectral-fluorescence characteristics of Ce6-PVP, Ce6-PEG, Ce6-BSA, Ce6-TX-100 indicate disaggregation of chlorine e6 molecules and their consecutive interaction with excipients in solutions and formation of molecular associates and molecular complexes. The system Ce6-chitosan is characterized by aggregation of pigments in solution that reduces photochemical activity of a photosensitizer. The fluorescence quantum yield jk of mentioned above supramolecular systems has been calculated. The results can be useful during the new method of controlled aggregation of photosensitizers as a part of supramolecular complexes development as well as for the purposes of new medicines of predictable photodynamic activity creation.
1. A. B. Uzdensky, Cellular and molecular mechanisms of photodynamic therapy, Science, SPb, Russia (2010).
2. A. L. Akopov, N. V. Kazakov, A. A. Rusanov, and A. Karlson, “The mechanisms of photodynamic action for treating of cancer patients,” Photodynamic therapy and photodiagnostics 4(2), 9-16 (2015) [in Russian].
3. E. V. Pinyaskina, “Photodynamic processes in cells. Primary mechanisms,” Bulletin of DSPU 1, 60-67 (2010) [in Russian].
4. E. Zenkevich, E. Sagun, V. Knyukshto, A. Shulga, A. Mironov, O. Efremova, R. Bonnett, S. P. Songca, and M. Kassem, “Photophysical and photochemical properties of potential porphyrin and chlorin photosensitizers for PDT,” J. of Photochemistry and Photobiology B: Biology 33(2) 171-180 (1996).
5. M. V. Parkhats, V. A. Galievsky, A. S. Stashevsky, T. V. Trukhacheva, and B. M. Dzhagarov, “Dynamics and efficiency of the photosensitized singlet oxygen formation by chlorin e6: the effects of the solution pH and polyvinylpyrrolidone,” Optics and Spectroscopy 107(6), 974–980 (2009).
6. C. T. H. Yen, G. V. Ramenskaya, and N. A. Oborotova, “Chlorin derivatives in cancer photodynamic therapy,” Rus. J. of Biotherapy 8(4), 99-104 (2009) [in Russian].
7. M. V. Parkhats, V. N. Knyukshto, H. A. Isakau, P. T. Petrov, and B. M. Dzhagarov, “Photophysical properties of photosensitizer chlorin e6 incorporated into polyvinylpyrrolidone,” Proc. SPIE 6727, 67272L (2007).
8. T. E. Zorina, I. V. Yankovsky, I. E. Kravchenko, T. V. Shman, M. V. Belevtsev, and V. P. Zorin, “Evaluation of Phototoxicity and cytotoxicity for chlorin e6 ester derivatives and their liposomal forms,” Biophysics 60(5), 759-766 (2015).
9. E. A. Genina, Biophotonics methods: Phototherapy, New Wind, Saratov, Russia (2012).
10. H. A. Isakau, M. V. Parkhats, V. N. Knyukshto, B. M. Dzhagarov, E. P. Petrov, and P. T. Petrov, “Toward understanding the high PDT efficacy of chlorin e6–polyvinylpyrrolidone formulations: Photophysical and molecular aspects of photosensitizer–polymer interaction in vitro,” J. of Photochem. and Photobiol. B: Biology 92(3), 165-174 (2008).
11. A. K. Visheratina, А. О. Orlova, V. G. Maslov, A. V. Fedorov, and A. V. Baranov, “Optical properties of conjugates of CdSe/ZnS quantum dots and chlorin e6 in aqueous solution,” J. of Opt. Tech. 82(11), 738-742 (2015).
12. S. L. Fiedaruk, T. V. Trukhacheva, S. N. Sokolov, K. A. Frolenko, and V. P. Kheidorov, “The study of spectral properties of dimethylester of chlorin e6 in the presence of polyvinilpyrrolidone,” Vestnik farmacii 3(65), 82-88 (2014) [in Russian].
13. V. Yu. Plavskii, V. A. Mostovnikov, G. R. Mostovnikova, A. I. Tret'yakova, and A. V. Mikulich, “Spectral-luminescent properties of chlorin e6 and malate dehydrogenase complexes,” J. of Appl. Spectroscopy 71(6), 818-828 (2004).
14. G. P. Gurinovich, A. I. Sevchenko, and K. N. Solov'ev, Spectroscopy of a chlorophyll and related compounds, Science and technology, Minsk, USSR (1978).
15. H. Podbielska, A. Ulatowska-Jarza, G. Muller, I. Holowacz, J. Bauer, and U. Bindig, “Silica sol-gel matrix doped with Photolon molecules for sensing and medical therapy purposes,” Biomolecular Engineering 24(5), 425-433 (2007).
16. A. A. Krasnovsky, “Photodynamic action and singlet oxygen,” Biophysics 49(2), 289-306 (2004) [in Russian].
17. G. V. Golovina, V. A. Ol'shevskaya, V. V. Vargin, V. A. Kuzmin, and A. B. Shevelev, “Solubility of porphyrine derivatives in vivo affected by aggregate formation and complexes formation with serum albumin,” Health of the population and habitat 12, 41-43 (2013) [in Russian].
18. N. H. Karapetyan, and V. N. Madakyan, “The Interaction of new pyridylporphyrins with bovine serum albumin,” Russ. J. of Boiorganic Chemistry 30(2), 172-177 (2004) [in Russian].
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