Study of blood microcirculation of pancreas in rats with alloxan diabetes by Laser Speckle Contrast Imaging

Polina A. Timoshina (Login required)
Research-Educational Institute of Optics and Biophotonics, Saratov National Research State University, Russia

Alla B. Bucharskaya
Saratov State Medical University, Russia

Denis A. Alexandrov
Saratov State Medical University, Russia

Valery V. Tuchin
Research-Educational Institute of Optics and Biophotonics, Saratov National Research State University, Russia
Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russia
Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Russia


Paper #3150 received 23 Jan 2017; revised manuscript received 10 Mar 2017; accepted for publication 13 Mar 2017; published online 29 Mar 2017.

DOI: 10.18287/JBPE17.03.020301

Abstract

The blood microcirculation of the pancreas in rats with diabetes was studied using Laser Speckle Contrast Imaging (LSCI). The impact on blood flow of x-ray contrast “OmnipaqueTM-300” (n = 1.438) and aqueous solution of “OmnipaqueTM-300” (n = 1.407) used as optical clearing agents (OCAs) was also investigated. The alloxan induced animal model of diabetes was exploited. The results obtained in the study of blood microcirculation disorders of pancreas in diabetes and under topical application of optical clearing agents show that disease development in animals causes changes in the microcirculatory system response to application of “OmnipaqueTM-300” solutions.

Keywords

speckle; blood flow; speckle contrast; diabet

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