Study of glycerol diffusion in skin and myocardium ex vivo under the conditions of developing alloxan-induced diabetes

Daria K. Tuchina, Alexey N. Bashkatov, Alla B. Bucharskaya, Elina A. Genina, Valery V. Tuchin


Diabetes mellitus is one of widespread diseases, the development of which impairs the functioning of organism vital organs. The study of the processes that occur in tissues under diabetic conditions facilitates the development of diabetes diagnostics and treatment methods, as well as the prognosis of its complications. The paper reports a comparative study of glycerol diffusion in skin and myocardium of rats ex vivo in the normal condition and the conditions of alloxan-induced diabetes. The glycerol diffusion coefficients in tissues were determined basing on the analysis of kinetics of collimated light transmittance through tissue samples immersed in 70%-glycerol solution. As a result, it was shown that the glycerol diffusion rate in the tissues of rats with alloxan-induced diabetes is essentially decreased as compared to the control group.


Skin; Myocardium; Diffusion coefficient; Permeability coefficient; Glycerol; Alloxan-induced diabetes; Collimated transmittance; Optical clearing

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