Raman and autofluorescence analysis of human body fluids from patients with malignant tumors

Lyudmila A. Shamina, Ivan A. Bratchenko, Dmitry N. Artemyev, Oleg O. Myakinin, Alexander A. Moryatov, Oleg I. Kaganov, Andrey E. Orlov, Sergey V. Kozlov, Valery P. Zakharov

Abstract


In this study we measured Raman and autofluorescence spectral features of blood and urine from patients with various cancers. A total number of 26 blood samples from patients with lung cancer and 12 blood samples from patients with other cancers, and also 10 urine samples from patients with lung cancer and 9 urine samples from patients with other tumors were tested. The processing of experimental data and definition of informative bands for body fluid spectral analysis were performed on the bases of PLS-DA method. Wherein, there is no significant correlation between the most informative criteria for blood and urine. This fact shows that simultaneous study of blood and urine samples can increase the analysis informativeness. In general, the developed approach of body fluids analysis may become the basis of an inexpensive, quick and reliable method of lung cancer screening.


Keywords


Raman spectroscopy; autofluorescence; lung cancer; biofluidity homeostasis; blood; urine

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