Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Yu-Chung Lin
Department of Physics, National Dong Hwa University, Hualien, Taiwan
Institute of Physics, Academia Sinica, Taipei, Taiwan

Zhe-Rui Lin
Department of Physics, National Dong Hwa University, Hualien, Taiwan

Lin-Wei Tsai
Department of Physics, National Dong Hwa University, Hualien, Taiwan

Elena Perevedentseva
Department of Physics, National Dong Hwa University, Hualien, Taiwan
P. N. Lebedev Physics Institute of Russian Academy of Science, Moscow, Russia

Artashes Karmenyan
Department of Physics, National Dong Hwa University, Hualien, Taiwan

Chia-Liang Cheng (Login required)
Department of Physics, National Dong Hwa University, Hualien, Taiwan

Paper #3162 received 27 Feb 2017; revised manuscript received 17 Apr 2017; accepted for publication 19 Apr 2017; published online 30 Apr 2017.

DOI: 10.18287/JBPE17.03.010305


The natural isoquinoline alkaloid berberine has been demonstrated for its significant activity against a variety of deceases caused by bacterial and parasite infections. Nanodiamond (ND) has also been proven to have great potential for drug delivery. In this work, the complex of ND with berberine is constructed through surface functionalized ND, aiming for drug delivery applications. Spectroscopic analysis was performed on the complex interaction with human lung alveolar carcinoma epithelial cell (A549) and red blood cell (RBC) in comparison with berberine in solution. The studied cells were treated with carboxylated ND, ND-berberine complex and berberine, respectively. Their distributions in cells structures are visualized using ND and berberine’s fluorescence signals for detection. The distribution of ND and ND-berberine complex differs with the distribution of berberine introduced in solution form. The results show that the ND with attached drug molecules can act as a drug carrier allowing controllable localization of the drug into the cell. This result can have implication in drug delivery, release and delivery tracing applications.


Nanodiamond; Berberine; Bio-Applications; Medical Applications; Fluorescence; Surface Functionality; Imaging; Drug delivery; Nanobiotechnology

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