Laser Illumination in Live Cell Microscopy: Scattering and Structured Illumination

Herbert Schneckenburger, Verena Richter, Mathis Piper, Michael Wagner


Two types of laser illumination in live cell microscopy with a focus on the sample or in the aperture plane of the microscope objective lens are distinguished. For the second case two examples are described, namely light scattering microscopy with angular resolution and Structured Illumination Microscopy (SIM) with two interfering laser beams. Appropriate applications include morphological studies of cells undergoing apoptosis and mitochondrial imaging with increased resolution.


Living cells; light scattering microscopy; super-resolution microscopy; structured illumination; SIM; apoptosis; mitochondrial imaging

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