Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues
Paper #3264 received 30 Nov 2017; revised manuscript received 17 Dec 2017; accepted for publication 18 Dec 2017; published online 31 Dec 2017. [Saratov Fall Meeting 2017 Special Issue].
New more sensitive diagnostic modalities, for either analysis of biopsy tissue samples or on site, in vivo microscopy tissue examination, emerge to aid the detection and accurate diagnosis of gastrointestinal cancer. One of the most intensively investigated techniques is the fluorescence spectroscopy approach.
We present our investigation of the detailed macro and micro fluorescence characteristics of ex-vivo fresh and fixed tissue samples. The fluorescence spectra of unprocessed cancerous and healthy gastrointestinal (GIT) tissue, obtained with two approaches fluorescence and synchronous fluorescence spectroscopy were compared with microspectroscopy performed with laser scanning confocal fluorescence microscopy (LSCFM) system in Lambda-scan regime over fixed tissue samples.The evaluated optical macro- and micro- spectroscopy characteristics are presented and disscused in order to improve the current understanding of the origin of autofluorescence contrast between healthy and cancerous tissues and its application for improvement of the existing diagnostic modalities.
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