Study of the Changes of Gastric Wall Mucosa Optical Properties under the Impact of Aqueous Solutions of Haemoglobin and Glucose for Improving Conditions of the Laser Coagulation

Alexey N. Bashkatov
Saratov State University, Russia
National Research Tomsk State University, Russia

Elina A. Genina (Login required)
Saratov State University, Russia
National Research Tomsk State University, Russia

Veniamin A. Grishaev
Saratov City Clinical Hospital No. 6 named after Acad. V.N. Koshelev, Russia

Sergey V. Kapralov
Engels City Clinical Hospital No. 1, Saratov Region, Russia

Vyacheslav I. Kochubey
Saratov State University, Russia
National Research Tomsk State University, Russia

Valery V. Tuchin
Saratov State University, Russia
National Research Tomsk State University, Russia
Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russia


Paper #3272 received 6 Dec 2017; revised manuscript received 18 Dec 2017; accepted for publication 19 Dec 2017; published online 31 Dec 2017. [Saratov Fall Meeting 2017 Special Issue].

DOI: 10.18287/JBPE17.03.040304

Abstract

The paper presents the results of ex vivostudies of the possibility to control the absorption and scattering properties of the human gastric wall mucosa. For the first time we obtained the increase of the tissue absorption coefficient in the range 350-1250 nm by 2-4.5 times under the injection of haemoglobin solution with the concentration of 70 g/L into the mucosa. The observed increase of the absorbed energy fraction by nearly 65-90% was accompanied by almost 50-60% decrease of the penetration depth at the wavelengths of the sources widely used for laser ablation and coagulation of gastric mucosa paraplasms. Under the injection of 40% glucose solution into the mucosa, we observed the reduction of the absorption coefficient in the spectral region of water absorption bands approximately by 20% and the reduction of the transport scattering coefficient by nearly 24-27% in the spectral range 350-2500 nm. Increasing of the depth of laser radiation penetration in this case amounted to 15-17% in the range 800-1100 nm. The performed studies show the possibility in principle to control the optical parameters of the gastric wall tissues from the point of view of both varying the laser radiation penetration depth and regulating the tissue absorbance and, hence, the power of laser radiation, which, in turn, will allow more safety and less invasion in the course of laser therapeutic procedures.

 

Keywords

Mucose; spectroscopy; optical properties control

Full Text:

PDF

References


1. C. T. Germer, D. Albrecht, C. Isbert, J. Ritz, A. Roggan, and H. J. Buhr, “Diffusing fibre tip for minimally invasive treatment of liver tumours by interstitial laser coagulation (ILC): an experimental ex vivo study,” Lasers in Medical Science 14, 32-39 (1999). Crossref

2. R. Sroka, C. G. Schmedt, S. Steckmeier, O. A. Meissner, W. Beyer, G. Babaryka, and B. Steckmeier, “Ex-vivo investigation of endoluminal vein treatment by means of radiofrequency and laser irradiation,” Medical Laser Application 21, 15-22 (2006). Crossref

3. C. M. Cilip, A. E. Ross, J. P. Jarow, and N. M. Fried, “Application of an optical clearing agent during noninvasive laser coagulation of the canine vas deferens,” Journal of Biomedical Optics 15(4), 048001 (2010). Crossref

4. G. R. Schweinsberger, C. M. Cilip, S. R. Trammell, H. Cherukuri, and N. M. Fried, “Noninvasive laser coagulation of the human vas deferens: optical and thermal simulations,” Lasers in Surgery and Medicine 43, 443-449 (2011). Crossref

5. J. T. Au, A. Mittra, J. Wong, S. Carpenter, J. Carson, D. Haddad, S. Monette, P. Ezell, S. Patel, and Y. Fong, “Flexible CO2 laser and submucosal gel injection for safe endoluminal resection in the intestines,” Surgical Endoscopy 26(1), 47-52 (2012). Crossref

6. S. Kim, M. T. Hossain, D. H. Lee, and J. K. Kim, “Analysis of opto-thermal interaction of porcine stomach tissue with 808-nm laser for endoscopic submucosal dissection,” Journal of Innovative Optical Health Sciences 8(6), 1550043 (2015) Crossref

7. V. Rubtsov, Yu. Chalyk, and A. Bashkatov, “Rational choice of laser wavelength for endoscopic photodestruction of colorectal polyps,” Vrach 12, 83-85 (2013) [in Russian].

8. J.-H. Cho, J. Y. Cho, M.-Y. Kim, S. R. Jeon, T. H. Lee, H. G. Kim, S. Y. Jin, and S. J. Hong, “Endoscopic submucosal dissection using a thulium laser: Preliminary results of a new method for treatment of gastric epithelial neoplasia,” Endoscopy 45(9), 725-728 (2013). Crossref

9. V. V. Tuchin, “Tissue optics and photonics: light-tissue interaction,” Journal of Biomedical Photonics & Engineering 1(2), 98-134 (2015). Crossref

10. T. Uraoka, T. Fujii, Y. Saito, T. Sumiyoshi, F. Emura, P. Bhandari, T. Matsuda, K. Fu, and D. Saito, “Effectiveness of glycerol as a submucosal injection for EMR,” Gastrointestinal Endoscopy 61, 736-740 (2000). Crossref

11. H. Yamamoto, H. Kawata, K. Sunada, K. Satoh, Y. Kaneco, K. Ido, and K. Sugano, “Success rate of curative endoscopic mucosal resection with circumferential mucosal incision assisted by submucosal injection of sodium hyaluronate,” Gastrointestinal Endoscopy 56, 507-512 (2002). Crossref

12. I. Kumano, M. Ishihara, S. Nakamura, S. Kishimoto, M. Fujita, H. Hattori, T. Horio, Y. Tanaka, K. Hase, and T. Maehara, “Endoscopic submucosal dissection for pig esophagus by using photocrosslinkable chitosan hydrogel as submucosal fluid cushion,” Gastrointestinal Endoscopy 75(4), 841-848 (2012). Crossref

13. A. N. Bashkatov, E. A. Genina, V. I. Kochubey, A. A. Gavrilova, S. V. Kapralov, V. A. Grishaev, and V. V. Tuchin, “Optical properties of human stomach mucosa in the spectral range from 400 to 2000 nm: prognosis for gastroenterology,” Medical Laser Application 22, 95-104 (2007). Crossref

14. K. M. Giraev, N. A. Ashurbekov, and O. V. Kobsev, “Optical spectra of some pathological conditions of stomach tissues,” International Journal of Modern Physics B 20(1), 25-36 (2006). Crossref

15. K. M. Giraev, N. A. Ashurbekov, and M. A. Lakhina, “Optical absorption and scattering spectra of pathological stomach tissues,” Journal of Applied Spectroscopy 78(1), 95-102 (2011). Crossref

16. H. J. Wei, D. Xing, B. H. He, H. M. Gu, G. Y. Wu, and X. M. Chen, “Using of oblique incident laser beam to measure the optical properties of stomach mucosa/submucosa tissue”, BMC Gastroenterology 9, 64, (2009) Crossref

17. R. K. Wang, X. Xu, Y. He, and J. B. Elder, “Investigation of optical clearing of gastric tissue immersed with hyperosmotic agents,” IEEE Journal of Selected Topics in Quantum Electronics 9(2), 234-242 (2003). Crossref

18. X. Xu, R. Wang, and J. B. Elder, “Optical clearing effect on gastric tissues immersed with biocompatible chemical agents investigated by near infrared reflectance spectroscopy,” Journal of Physics D: Applied Physics 36, 1707-1713 (2003). Crossref

19. Y. He, and R. K. Wang, “Dynamic optical clearing effect of tissue impregnated with hyperosmotic agents and studied with optical coherence tomography,” Journal of Biomedical Optics 9(1), 200-206 (2004). Crossref

20. X. Xu, and R. K. Wang, “Synergistic effect of hyperosmotic agents of dimethyl sulfoxide and glycerol on optical clearing of gastric tissue studied with near infrared spectroscopy,” Physics in Medicine & Biology 49, 457-468 (2004). Crossref

21. H. Xiong, Z. Guo, C. Zeng, L. Wang, Y. He, and S. Liu, “Application of hyperosmotic agent to determine gastric cancer with optical coherence tomography ex vivo in mice,” Journal of Biomedical Optics 14(2), 024029 (2009). Crossref

22. V. V. Tuchin, D. M. Zhestkov, A. N. Bashkatov, and E. A. Genina, “Theoretical study of immersion optical clearing of blood in vessels at local hemolysis,” Optics Express 12(13), 2966-2971 (2004). Crossref

23. S. A. Prahl, M. J. C. van Gemert, and A. J. Welch, “Determining the optical properties of turbid media by using the adding-doubling method,” Applied Optics 32(4), 559-568 (1993). Crossref

24. A. N. Bashkatov, E. A. Genina, V. I. Kochubey, V. S. Rubtsov, E. A. Kolesnikovа, and V. V. Tuchin, “Optical properties of human colon tissue in the 350 – 2500 nm spectral range,” Quantum Electronics 44(8), 779-784 (2014). Crossref

25. A. N. Bashkatov, E. A. Genina, M. D. Kozintseva, V. I. Kochubei, S. Yu. Gorodkov, and V. V. Tuchin, “Optical properties of peritoneal biological tissues in the spectral range of 350–2500 nm,” Optics and Spectroscopy 120(1), 1-8 (2016). Crossref

26. L. Wang, S. L. Jacques, and L. Zheng, “MCML – Monte Carlo modeling of light transport in multi-layered tissues,” Computer Methods and Programs in Biomedicine 47, 131-146 (1995). Crossref

27. W.H. Press, S. A. Tuekolsky, W. T. Vettering, and B. P. Flannery, “Numerical recipes in C: The art of scientific computing,” Cambridge University Press, Cambridge, New York (1992). Crossref

28. V. V. Tuchin, “Tissue optics: Light Scattering Methods and Instruments for Medical Diagnosis. Third edition,” SPIE Press, Washington, Bellingham (2015). Crossref






© 2014-2018 Samara National Research University. All Rights Reserved.
Public Media Certificate (RUS). 12+