Estimation of dehydration of skin by refractometric method using optical clearing agents

Ekaterina N. Lazareva (Login required)
Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russian Federation
Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Russian Federation

Polina A. Dyachenko (Timoshina)
Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russian Federation
Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Russian Federation

Alla B. Bucharskaya
Saratov State Medical University, Russian Federation

Nikita A. Navolokin
Saratov State Medical University, Russian Federation

Valery V. Tuchin
Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russian Federation
Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Russian Federation
Laboratory for Laser Diagnostics of Technical and Live Systems, Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russian Federation
Laboratory of Femtomedicine, ITMO University, St. Petersburg, Russian Federation
Samara National Research University, Russian Federation


Paper #3317 received 15 Feb2019; revised manuscript received 26 May 2019; accepted for publication 5 Jun 2019; published online 30 Jun 2019.

DOI: 10.18287/JBPE19.05.020305

Abstract

Optical methods with highly sensitive to water content are important for the development of new diagnostic and therapeutic methods in medicine. The paper presents a quantitative assessment of the dehydration of skin when using optical clearing agents (glycerol and glucose) by the method of multi-wavelength refractometry for the visible and NIR spectral regions. The resulting decrease in the refractive index of the optical clearing solution made it possible to estimate the volume of the fluid extracted from the tissue. The possibility of using the method when conducting in vivomeasurements is shown. An assessment was made of the degree of dehydration of rat skin in areas with a subcutaneous tumor neoplasm, which was three times less than for control (healthy) skin areas.

Keywords

multi-wavelength refractometry; skin; refractive index; dehydration; optical clearing; glycerol; glucose

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References


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