Monte Carlo simulation of skin multispectral autofluorescence

Anastasia O. Ustinova
Samara National Research University, Russia

Ivan A. Bratchenko
Samara National Research University, Russia

Dmitry N. Artemyev orcid (Login required)
Samara National Research University, Russia

Paper #3326 received 2 May 2019; revised manuscript received 8 Jun 2019; accepted for publication 15 Jun 2019; published online 30 Jun 2019.

DOI: 10.18287/JBPE19.05.020306


This work is devoted to the simulation of human skin autofluorescence in different spectral ranges. Analytical review was performed for selecting the main endogenous fluorophores with the greatest contribution to the skin fluorescence: tryptophan, tyrosine, collagen, melanin, elastin, lipofuscin, protoporphyrin IX, NADH, FAD. It was necessary to set parameters for autofluorescence modeling, such as the absorption/emission spectra of fluorophores, molar concentration, molar extinction coefficient, and quantum yield. The six-layer skin model was designed in the TracePro software and autofluorescence was simulated when excited at different wavelengths in the middle UV (270-300 nm), near UV (330-360 nm) and visible (400-450 nm) spectral ranges. The simulation results were compared with the experimental results of other authors. The principal distinctive factor of this work is the simulation of the human skin autofluorescence excited in different spectral ranges.


autofluorescence; Monte Carlo modeling; skin model; skin fluorophores; optical diagnostics

Full Text:



1. A. C. Croce, G. Bottiroli, “Autofluorescence spectroscopy and imaging: A tool for biomedical research and diagnosis,” European Journal of Histochemistry 58(4), (2014). Crossref

2. V. V. Dremin, A. V. Dunaev, “How the melanin concentration in the skin affects the fluorescence-spectroscopy signal formation,” Journal of Optical Technology 83(1), 43–48 (2016). Crossref

3. E. G. Borisova, L. P. Angelova, and E. P. Pavlova, “Endogenous and Exogenous Fluorescence Skin Cancer Diagnostics for Clinical Applications,” IEEE Journal of Selected Topics in Quantum Electronics 20(2), 211–222 (2014). Crossref

4. P. Meredith, J. Riesz, “Radiative Relaxation Quantum Yields for Synthetic Eumelanin,” Photochemistry and Photobiology 79(2), 211-216 (2004). Crossref

5. V. V. Tuchin, Tissue Optics, Light Scattering Methods and Instruments for Medical Diagnostics, Third Edition, SPIE, Bellingham (2007). ISBN: 9781628415162.

6. M. A. Ansari, E. Mohajerani, “Mechanisms of Laser-Tissue Interaction: I. Optical Properties of Tissue,” Journal of Lasers in Medical Sciences 2(3), 119-125 (2011). Crossref

7. G. D. Fasman, Handbook of Biochemistry and Molecular Biology, Third Edition, Ohio CRC Press, Cleveland (1976). ISBN 9780367260903.

8. R. F. Chen, “Measurements of absolute values in biochemical fluorescence spectroscopy,” Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry 76A(6), 593-606 (1972). Crossref

9. I. Meglinski, Biophotonics for Medical Applications, Elsevier, Amsterdam (2014). ISBN: 9780857096623.

10. Collagen alpha-1(I) chain, Protein A005366, Signaling Gateway (date of access: 29.04.2018).

11. Elastin, Protein A002955, Signaling Gateway (date of access: 29.04.2018).

12. J. Tombran-Tink, C. J. Barnstable, Retinal Degenerations: Biology, Diagnostics, and Therapeutics, Humana Press, Totowa, NJ (2007). ISBN 9781597451864.

13. J. R. Sparrow, Y. Wu, C. Y. Kim, and J. Zhou, “Phospholipid meets all-trans-retinal: the making of RPE bisretinoids,” Journal of Lipid Research 51(2), 247–261 (2009). Crossref

14. M. Gouterman, G.-E. Khalil, “Porphyrin free base phosphorescence,” Journal of Molecular Spectroscopy 53(1), 88-100 (1974). Crossref

15. K. S. Brandenburg, K. J. Rodriguez, J. F. McAnulty, C. J. Murphy, N. L. Abbott, M. J. Schurr, and C. J. Czuprynski, “Tryptophan inhibits biofilm formation by Pseudomonas aeruginosa,” Antimicrobial Agents and Chemotherapy 57(4), 1921–1925 (2013). Crossref

16. L. Brancaleon, G. Lin, and N. Kollias, “The In Vivo Fluorescence of Tryptophan Moieties in Human Skin Increases with UV Exposure and is a Marker for Epidermal Proliferation,” Journal of Investigative Dermatology 113(6), 977-982 (1999). Crossref

17. D. M. Jameson, “A Fluorescent Lifetime: Reminiscing About Gregorio Weber,” Chap in Perspectives on Fluorescence, 1-16 (2016). Crossref

18. E. Borisova, P. Pavlova, E. Pavlova, P. Troyanova, and L. Avramov, “Optical Biopsy of Human Skin – A Tool for Cutaneous Tumours’ Diagnosis,” International Journal Bioautomation 16(1), 53-72 (2012).

© 2014-2019 Samara National Research University. All Rights Reserved.
Public Media Certificate (RUS). 12+