Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Anastasia I. Maslianitsyna orcid (Login required)
Physics faculty, Lomonosov Moscow State University, Russian Federation

Petr B. Ermolinsky
Physics faculty, Lomonosov Moscow State University, Russian Federation

Andrei E. Lugovtsov
Physics faculty, Lomonosov Moscow State University, Russian Federation
International Laser Centre, Lomonosov Moscow State University, Russian Federation

Alexander V. Priezzhev
Physics faculty, Lomonosov Moscow State University, Russian Federation
International Laser Centre, Lomonosov Moscow State University, Russian Federation


Paper #3371 received 19 May 2020; revised manuscript received 15 Jun 2020; accepted for publication 19 Jun 2020; published online 22 Jun 2020.

DOI: 10.18287/JBPE20.06.020305

Abstract

Blood microcirculation in human body is greatly dependent on the microrheologic properties of red blood cells. The aim of this work is to identify the relationship between the deformability of these cells and their aggregation properties, both of which are the key factors for the blood flow. Laser diffractometry, diffuse light scattering and laser tweezers were implemented for in vitromeasurements. Different osmolarity of plasma (150–500 mOsm/l) and concentrations of glutaraldehyde (up to 0.004%) were used to change the deformability of healthy red blood cells in vitro. The results show that with the cells becoming more rigid some aggregation parameters (e.g. the fraction of aggregated cells) decrease, while some of them (e.g. the hydrodynamic strength of the aggregate) stay unchanged. For example, after incubation in 0.004% glutaraldehyde solution the erythrocyte deformability drops by 19 ± 2% and this leads to a decrease by 77 ± 4% in the aggregation index. This means that there is a connection between cell deformability and the formation of the aggregates, however the relationship is less pronounced and more complex for the disaggregation process.

Keywords

blood; erythrocytes; red blood cell aggregation; deformability; laser diffractometry; laser tweezers; diffuse light scattering; glutaraldehyde; osmolarity

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