UV-NIR efficiency of the refractive index matching mechanism on colorectal muscle during treatment with different glycerol osmolarities

Nelma Gomes
Department of Physics, Polytechnic of Porto – School of Engineering, Porto, Portugal

Valery V. Tuchin orcid
Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russia
Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov, Russia
Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Russia

Luís M. Oliveira orcid (Login required)
Department of Physics, Polytechnic of Porto – School of Engineering, Porto, Portugal
Centre of Innovation in Engineering and Industrial Technology, Porto, Portugal


Paper #3374 received 25 May 2020; revised manuscript received 12 Jun 2020; accepted for publication 14 Jun 2020; published online 26 Jun 2020.

DOI: 10.18287/JBPE20.06.020307

Abstract

The evaluation of the optical clearing mechanisms in tissues provides information about the efficiency of the clearing treatment. One of such mechanisms is the refractive index matching, which is created by the partial replacement of tissue water by an optical clearing agent with higher refractive index, better matched to the index of tissue scatterers. With the objective of evaluating the refractive index matching mechanism for a wide spectral range and comparing its magnitude between treatments with different clearing agent osmolarities, thickness and collimated transmittance measurements were obtained from human colorectal muscle samples under treatment with 20%-, 40% and 60%-glycerol. Such measurements were used in a calculation model to obtain the refractive index kinetics for the interstitial fluid and for the whole tissue. The calculation results show that the refractive index matching has a stronger effect in the ultraviolet and that such matching is more effective for higher agent concentrations in the treating solutions.

Keywords

ultraviolet spectroscopy; refractive index matching; light scattering; tissue water content; optical clearing; colorectal muscle; collimated transmittance

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