Tissue Optical Clearing in the Ultraviolet for Clinical Use in Dentistry to Optimize the Treatment of Chronic Recurrent Aphthous Stomatitis

Alexey A. Selifonov (Login required)
Saratov State University, Russia
Children's Polyclinic No.3 of the Children's Infectious Diseases Clinical Hospital No.5, Saratov, Russia

Valery V. Tuchin
Saratov State University, Russia

National Research Tomsk State University, Russia
Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov, Russia


Paper #3394 received 15 Nov 2020; revised manuscript received 20 Dec 2020; accepted for publication 22 Dec 2020; published online 31 Dec 2020.

DOI: 10.18287/JBPE20.06.040301

Abstract

The immersion optical clearing (OC) treatment with a highly concentrated glycerol solution has induced three new tissue windows in the UV spectral range of gingival tissues – from 200 to 250 nm, from 250 to 300 nm and from 300 to 400 nm. By combining the immersion OC technique in human tissues with UV-spectroscopy, it was possible to verify and study the major OC mechanisms – tissue dehydration and refractive index matching, and that the OC efficiency is higher in the deep-UV than in the visible-NIR range. Since all biological tissues present high scattering in the UV range, the presented technology, which basically reduces the strong light scattering in the UV range, has a broad application area in medicine. The effectiveness of the developed technology combining UV phototherapy and OC in application to treatment of aphthous recurrent stomatitis in children was demonstrated.

Keywords

immersion optical clearing; UV spectrum; human gingiva; aphthous recurrent stomatitis

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