Tissue Optical Clearing in the Ultraviolet for Clinical Use in Dentistry to Optimize the Treatment of Chronic Recurrent Aphthous Stomatitis

Alexey A. Selifonov (Login required)
Saratov State University, Russia
Children's Polyclinic No.3 of the Children's Infectious Diseases Clinical Hospital No.5, Saratov, Russia

Valery V. Tuchin
Saratov State University, Russia

National Research Tomsk State University, Russia
Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov, Russia

Paper #3394 received 15 Nov 2020; revised manuscript received 20 Dec 2020; accepted for publication 22 Dec 2020; published online 31 Dec 2020.

DOI: 10.18287/JBPE20.06.040301


The immersion optical clearing (OC) treatment with a highly concentrated glycerol solution has induced three new tissue windows in the UV spectral range of gingival tissues – from 200 to 250 nm, from 250 to 300 nm and from 300 to 400 nm. By combining the immersion OC technique in human tissues with UV-spectroscopy, it was possible to verify and study the major OC mechanisms – tissue dehydration and refractive index matching, and that the OC efficiency is higher in the deep-UV than in the visible-NIR range. Since all biological tissues present high scattering in the UV range, the presented technology, which basically reduces the strong light scattering in the UV range, has a broad application area in medicine. The effectiveness of the developed technology combining UV phototherapy and OC in application to treatment of aphthous recurrent stomatitis in children was demonstrated.


immersion optical clearing; UV spectrum; human gingiva; aphthous recurrent stomatitis

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1. V. V. Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnostics, SPIE Press, Bellingham (2015).

2. C. Sicora, A. Szilárd, L. Sass, E. Turcsányi, Z. Máté, and I. Vass, “UV-B and UV-A Radiation Effects on Photosynthesis at the Molecular Level,” Chapter in: Environmental UV Radiation: Impact on Ecosystems and Human Health and Predictive Models. Nato Science Series: IV: Earth and Environmental Sciences, F. Ghetti, G. Checcucci, J. F. Bornman (eds), Springer, Dordrecht, 121–135 (2006).

3. L. Shi, R. R. Alfano, Deep Imaging in Tissue and Biomedical Materials: Using Linear and Nonlinear Optical Methods, CRC Press, Boca Raton (2017).

4. L. Oliveira, V. V. Tuchin, The Optical Clearing Method: A New Tool for Clinical Practice and Biomedical Engineering, Basel, Springer Nature Switzerland AG (2019).

5. B. Baumann, “Polarization sensitive optical coherence tomography: a review of technology and applications,” Applied Sciences 7(5), 474 (2017).

6. Z. Hamdoon, W. Jerjes, G. McKenzie, A. Jay, and C. Hopper, “Optical coherence tomography in the assessment of oral squamous cell carcinoma resection margins,” Photodiagnosis and Photodynamic Therapy 13, 211–217 (2016).

7. N. M. Le, Sh. Song, H. Zhou, J. Xu, Y. Li, C.-E. Sung, A. Sadr, K.-H. Chung, H. M. Subhash, L. Kilpatrick, and R. K. Wang, “A noninvasive imaging and measurement using optical coherence tomography angiography for the assessment of gingiva: An in vivo study,” Journal of Biophotonics 11(12), e201800242 (2018).

8. M.-T. Tsai, Y. Chen, Ch.-Y. Lee, B.-H. Huang, N. H. Trung, Y.-J. Lee, and Y.-Li Wang, “Noninvasive structural and microvascular anatomy of oral mucosae using handheld optical coherence tomography,” Biomedical Optics Express 8(11), 5001–5012 (2017).

9. M. M. Perez, R. Ghinea, L. J. Herrera, F. Carrillo, A. M. Ionescu, and R. D. Paravina, “Color difference thresholds for computer-simulated human gingival,” Journal of Esthetic and Restorative Dentistry 30(2), E24–30 (2018).

10. I. Sailer, “Threshold values for the perception of color changes in human teeth,” The International Journal of Periodontics & Restorative Dentistry 36(6), 777–783 (2016).

11. N. D. Sarmast, N. Angelov, R. Ghinea, J. Powers, and R. Paravina, “Color compatibility of gingival shade guides and gingiva-colored dental materials with healthy human gingival,” The International Journal of Periodontics & Restorative Dentistry 38(3), 397–403 (2018).

12. D. K. Ho, R. Ghinea, L. J. Herrera, N. Angelov, and R. D. Paravina, “Color range and color distribution of healthy human gingiva: a prospective clinical study,” Scientific Reports 5(1), 18498 (2015).

13. V. V. Tuchin, Optical Clearing of Tissues and Blood, SPIE Press, Bellingham, USA (2005).

14. H. Kang, C. L. Darling, and D. Fried, “Use of an optical clearing agent to enhance the visibility of subsurface structures and lesions from tooth occlusal surfaces,” Journal of Biomedical Optics 21(8), 081206 (2016).

15. J. B. Schutt, J. F. Arens, C. M. Shai, and E. Stromberg, “Highly Reflecting Stable White Paint for the Detection of Ultraviolet and Visible Radiations,” Applied Optics 13(10), 2218–2221 (1974).

16. Refractive Index Database (accessed 14 December 2020).

17. A. Vogel, V. Venugopalan, “Mechanisms of pulsed laser ablation of biological tissues,” Chemical Reviews 103(2), 577–644 (2003).

18. I. Carneiro, S. Carvalho, R. Henrique, L. Oliveira, and V. Tuchin, “Moving tissue spectral window to the deep-ultraviolet via optical clearing,” Journal of Biophotonics 12(12), e201900181 (2019).

19. N. Altenburg, N. El-Haj, C. Micheli, M. Puttkammer, M. Abdel-Naser, and C. C. Zouboulis, “The Treatment of Chronic Recurrent Oral Aphthous Ulcers,” Deutsches Aerzteblatt Online 111(40), 665–673 (2014).

20. B. I. Tarakji, G. Gazal, S. Ali Al-Maweri, S. N. Azzeghaiby, and N. Alaizari “Guideline for the Diagnosis and Treatment of Recurrent Aphthous Stomatitis for Dental Practitioners,” Journal of International Oral Health 7(5), 74–80 (2015).

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