Combined Fluorescence and Bright Field Imaging via a Single CMOS Detector Without Filters to Improve on Patient Safety during Endoscopic Procedures – An Experimental Study Analysing the Output of both LASER and LED Near Infrared Sources on In Vitro Samples

Angharad Curtis (Login required)
Wireless & Optoelectronics Research & Innovation Centre, Faculty of Computing, Engineering and Science, University of South Wales, Pontypridd, UK

Kang Li
Wireless & Optoelectronics Research & Innovation Centre, Faculty of Computing, Engineering and Science, University of South Wales, Pontypridd, UK

Mohammed Ali Roula
School of Engineering, Faculty of Computing, Engineering and Science, University of South Wales, Pontypridd, UK

Nigel Copner
Wireless & Optoelectronics Research & Innovation Centre, Faculty of Computing, Engineering and Science, University of South Wales, Pontypridd, UK
Centre for Photonics Expertise, University of South Wales, Pontypridd, UK


Paper #3396 received 18 Nov 2020; revised manuscript received 19 Dec 2020; accepted for publication 20 Dec 2020; published online 31 Dec 2020.

DOI: 10.18287/JBPE20.06.040303

Abstract

Many endoscopic systems combine white light and NIR light via LED multiplexing in order to image tissue stained with Near Infrared (NIR) fluorescent markers such as Indocyanine Green (ICG). This can result in unnecessary in vivo energy exposure which can cause irreversible damage to tissue. A simple, single detector system is proposed in this work comprising a single excitation channel at a wavelength of 780 nm. We have demonstrated that a single 1.6 Megapixel CMOS camera with quantum efficiency of less than 30% is appropriate to capture both fluorescent and non-fluorescent landmarks at NIR wavelengths. Experimental results indicate that a LASER source generating between up to 10 mW of optical power at 780 nm could be considered as an alternative to LED.

Keywords

fluorescence endoscopy; fluorophore imaging; indocyanine green; illumination and detection

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