Influence of Activators and Inhibitors on the Collagenase with Collagen Interaction Monitored by Dynamic Light Scattering in Solutions

Irina A. Sergeeva
Chair of Molecular Processes and Extreme Matter States, Faculty of Physics, Lomonosov Moscow State University, Russian Federation

Anna V. Petrova orcid (Login required)
Chair of Molecular Processes and Extreme Matter States, Faculty of Physics, Lomonosov Moscow State University, Russian Federation

Anastasia V. Shlenskaya
Chair of Molecular Processes and Extreme Matter States, Faculty of Physics, Lomonosov Moscow State University, Russian Federation

Galina P. Petrova
Chair of Molecular Processes and Extreme Matter States, Faculty of Physics, Lomonosov Moscow State University, Russian Federation


Paper #3405 received 29 Jan 2021; revised manuscript received 1 May 2021; accepted for publication 13 May 2021; published online 2 Jun 2021.

DOI: 10.18287/JBPE21.07.020305

Abstract

Real–time digestion of type I collagen molecules by bacterial collagenase from Clostridium histolyticum has been monitored using Dynamic Light Scattering method. Time dependencies for translation diffusion coefficient (Dt) and hydrodynamic radius (RH) on were obtained for pure “collagen + collagenase” Tris–HCl buffer solution at different temperatures and for solutions with added CaCl2, ZnCl2 and MgCl2 and EDTA. It was shown that digestion of type I collagen molecules by bacterial collagenase is the first-order reaction. Reaction rate coefficients were calculated.

Keywords

dynamic light scattering; type I collagen; translation diffusion coefficient; collagenase from Clostridium histolyticum; first-order reaction; activators; inhibitors; reaction rate coefficient

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