Influence of Activators and Inhibitors on the Collagenase with Collagen Interaction Monitored by Dynamic Light Scattering in Solutions
Paper #3405 received 29 Jan 2021; revised manuscript received 1 May 2021; accepted for publication 13 May 2021; published online 2 Jun 2021.
Real–time digestion of type I collagen molecules by bacterial collagenase from Clostridium histolyticum has been monitored using Dynamic Light Scattering method. Time dependencies for translation diffusion coefficient (Dt) and hydrodynamic radius (RH) on were obtained for pure “collagen + collagenase” Tris–HCl buffer solution at different temperatures and for solutions with added CaCl2, ZnCl2 and MgCl2 and EDTA. It was shown that digestion of type I collagen molecules by bacterial collagenase is the first-order reaction. Reaction rate coefficients were calculated.
1. R. Bauer, K. Janowska, K. Taylor, B. Jordan, S. Gann, T. Janowski, E. C. Latimer, O. Matsushita, and J. Sakon, “Structures of three polycystic kidney disease-like domains from Clostridium histolyticum collagenases ColG and ColH,” Acta Crystallographica Section D: Biological Crystallography 71(3), 565–577 (2015).
2. I. Mandl, “Collagenases and elastases,” in Advances in Enzymology and Related Areas of Molecular Biology 23, F. F. Nord (Eds.), Interscience Publishers, New York, 163–264 (1961).
3. Collagenase from Clostridium histolyticum, Merck KGaA, (accessed 01 May 2021). [https://www.sigmaaldrich.com/catalog/product/sigma/c9891].
4. S. Müller-Herrmann, T. Scheibel, “Enzymatic degradation of films, particles, and nonwoven meshes made of a recombinant spider silk protein,” ACS Biomaterials Science & Engineering 1(4), 247–259 (2017).
5. J. Nanchahal, K.S. Midwood, “Treatment for dupuytren’s disease,” Isis Innovation Limited 8(2), 22–23 (2015).
6. M. F. Paige, A. C. Lin, and M. C. Goh, “Real-time enzymatic biodegradation of collagen fibrils monitored by atomic force microscopy,” International Biodeterioration & Biodegradation 50(1), 1–10 (2002).
7. S. K. Mallya, K. A. Mookhtiar, and H. E. Van Wart, “Kinetics of hydrolysis of type I, II, and III collagens by the class I and II Clostridium histolyticum collagenases,” Journal of Protein Chemistry 11(1), 99–107 (1992).
8. A. V. Petrova, I. A. Sergeeva, G. P. Petrova, and A. V. Mitrofanova, “The Features of Interaction of Collagen and Collagenase Molecules in the Presence of Chromium and Calcium Ions in Solutions by Dynamic Light Scattering,” Moscow University Physics Bulletin 75(6), 611–617 (2020).
9. R. Ritchie, W. Yang, and M. Meyers, “Structural Architectures with Toughening Mechanisms in Nature: A Review of the Materials Science of Type-I Collagenous Materials,” Progress in Materials Science 103, 425–483 (2019).
10. K. Wu, W. Liu, and G. Li, “The aggregation behavior of native collagen in dilute solution studied by intrinsic fluorescence and external probing,” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 102, 186–193 (2012).
11. Worthington Tissue Dissociation Guide, Worthington Biochemical Corporation, (accessed 01 May 2021). [http://www.worthington-biochem.com/tissuedissociation/collagenase.html].
12. N. Ohbayashi, N. Yamagata, M. Goto, K. Watanabe, Y. Yamagata, and K. Murayama, “Enhancement of the Structural Stability of Full-Length Clostridial Collagenase by Calcium Ions,” Applied and Enviromental Microbiology 78, 5839–5844 (2012).
13. J. J. Wilson, O. Matsushita, A. Okabe, and J. Sakon, “A Bacterial Collagen-binding Domain with Novel Calcium-binding Motif Controls Domain Orientation,” The EMBO Journal 22(8), 1743–52 (2003).
14. E. L. Angleton, H. E. Van Wart, “Preparation and Reconstitution with Divalent Metal Ions of Class I and Class II Clostridium histolyticum apocollagenases,” Biochemistry 27(19), 7406–7412 (1988).
15. S. Seifter, P. M. Gallop, LeRoy Klein, and E. Meilman, “Studies on Collagen: II. Properties of Purified Collagenase and Its Inhibition,” Journal of Biological Chemistry 234(1), 285-293 (1959).
16. P. Seglen, “Preparation of isolated rat liver cells,” Methods in Cell Biology 13, 29-83 (1976).
17. H. Z. Cummins, E. R. Paik (Eds.), Photon Correlation and Light Beating Spectroscopy, Springer Science and Business Media, New York (1974). ISBN: 978-1-4615-8906-8.
19. Multi-angle particle size analyzer Photocor Complex, Photocor Instrument, (accessed 01 May 2021). [http://www.photocor.com/dls-instrument].
20. P. Atkins, J. Paula, Physical Chemistry, 8-th ed., W. H. Freeman and Company, Gordonsville, USA (2006).
21. U. Eckhard, E. Schönauer, D. Nüss, and H. Brandstetter, “Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis,” Nature Structural & Molecular Biology 18(10), 1109–1115 (2011).
22. G. P. Robert, R. G. Pearson, “Absolute Hardness: Companion Parameter to Absolute Electronegativity,” Journal of the American Chemical Society 105(26), 7512–7516 (1983).
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