Estimation of Rabbit Pancreas Dispersion Between 400 and 1000 nm

Inês Martins
Physics Department, School of Engineering, Polytechnic Institute of Porto, Portugal
Center of Innovation in Engineering and Industrial Technology, ISEP, Porto, Portugal

Hugo Silva
Physics Department, School of Engineering, Polytechnic Institute of Porto, Portugal
Center of Innovation in Engineering and Industrial Technology, ISEP, Porto, Portugal

Valery V. Tuchin
Science Medical Center, Saratov State University, Russian Federation
Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Russian Federation
Laboratory of Laser Diagnostics of Technical and Living Systems, Precision Mechanics and Control Institute of the Russian Academy of Sciences, Saratov, Russian Federation

Luís Oliveira (Login required)
Physics Department, School of Engineering, Polytechnic Institute of Porto, Portugal
Center of Innovation in Engineering and Industrial Technology, ISEP, Porto, Portugal

Paper #3409 received 13 Mar 2021; revised manuscript received 8 Apr 2021; accepted for publication 12 Apr 2021; published online 7 May 2021.

DOI: 10.18287/JBPE21.07.020303


Current biophotonics methods cover the entire optical spectrum from the deep ultraviolet to the terahertz. To optimize such methods for diagnostic and therapeutic applications, the need to obtain the wideband dispersion of tissues is high. The pancreas is a very important organ in the human body, since it produces insulin and its malfunction may induce diabetes. A reduced number of biophotonics publications regarding the pancreas is available, meaning that studies to determine its optical properties and their variation during optical clearing treatments are necessary. Considering this fact, we used the total internal reflection method to measure the refractive index of the rabbit pancreas for wavelengths between 400 and 850 nm. The experimental results allowed to calculate the pancreas dispersion with the Cauchy, Conrady and Cornu equations. It was observed that all those equations provided good data fitting in the spectral range of the measurements, but differences were observed outside these limits. Considering the wavelength of 633 nm, the mean value from the three dispersions was 1.3521, while the one published for porcine pancreas is 1.3517. The dispersion calculated with the Conrady equation does not present a fast decreasing behavior for shorter wavelengths as the ones calculated with the Cauchy and Cornu equations, but comparing these curves with a dispersion for a tissue-like material, all seem to have good agreement.


pancreas tissue; refractive index; total internal reflection; tissue dispersion

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