Photodynamic Action in Thin Sensitized Layers: Estimating the Utilization of Light Energy

Gennady Meerovich (Login required)
Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia

Igor Romanishkin orcid
Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia

Ekaterina Akhlyustina
National Research Nuclear University 'MEPHI', Moscow, Russia

Marina Strakhovskaya
Department of Biology, Lomonosov Moscow State University, Russia
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies of the Federal Medical and Biological Agency of Russia, Moscow, Russia

Evgeniya Kogan
I. M. Sechenov First Moscow State Medical University, Russia

Ivan Angelov
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria
Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria

Victor Loschenov
Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
National Research Nuclear University 'MEPHI', Moscow, Russia

Ekaterina Borisova
Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria




DOI: 10.18287/JBPE21.07.040301

Abstract

The result of photodynamic action significantly depends on the density of the light dose absorbed by the photosensitizer. The efficiency of using light to excite photosensitizer molecules and minimization of its loss plays an important role in ensuring the overall success of the process. When carrying out photodynamic treatment of thin sensitized layers (such as inactivation of surface pathogens or in vitro screening studies of photosensitizers), only a part of the light dose is absorbed in the layer, while a significant part is lost, especially at low concentrations of the photosensitizer. In this work, we evaluate the decrease in absorbed light dose depending on the extinction and concentration of the photosensitizer in a thin sensitized layer, the shape of its absorption spectrum, and the shape of the excitation light source spectrum. It was found out that a significant loss of the absorbed dose occurs upon excitation of photosensitizers, especially with low extinction, when using light sources with a broad emission spectrum. This loss must be taken into consideration when predicting the results of photodynamic exposure and optimizing its tactics.

Keywords

photodynamic action; relative absorbed photodynamic dose; laser diode; photosensitizer.

Full Text:

PDF

References


1. J. Usuda, T. Inoue, T. Tsuchida, K. Ohtani, S. Maehara, N. Ikeda, Y. Ohsaki, T. Sasaki, and K. Oka, “Clinical trial of photodynamic therapy for peripheral-type lung cancers using a new laser device in a pilot study,” Photodiagnosis and Photodynamic Therapy 30, 101698 (2020).

2. H. Abrahamse, M. R. Hamblin, “New photosensitizers for photodynamic therapy,” Biochemical Journal 473(4), 347–364 (2016).

3. G. A. Meerovich, E. V. Akhlyustina, I. G. Tiganova, E. A. Lukyanets, E. A. Makarova, E. R. Tolordava, O. A. Yuzhakova, I. D. Romanishkin, N. I. Philipova, Yu. S. Zhizhimova, Yu. M. Romanova, V. B. Loschenov, and A. L. Gintsburg, “Novel Polycationic Photosensitizers for Antibacterial Photodynamic Therapy,” in Advances in Experimental Medicine and Biology, Springer, New York, 1–19 (2019).

4. M. C. Geralde, I. S. Leite, N. M. Inada, A. C. G. Salina, A. I. Medeiros, W. M. Kuebler, C. Kurachi, and V. S. Bagnato, “Pneumonia treatment by photodynamic therapy with extracorporeal illumination - an experimental model,” Physiological Reports 5(5), e13190 (2017).

5. L. D. Dias, V. S. Bagnato, “An update on clinical photodynamic therapy for fighting respiratory tract infections: a promising tool against COVID-19 and its co-infections,” Laser Physics Letters 17(8), 083001 (2020).

6. E. Ben-Hur, A. C. E. Moor, H. Margolis-Nunno, P. Gottlieb, M. M. Zuk, S. Lustigman, B. Horowitz, A. Brand, J. Van Steveninck, and T. M. A. R. Dubbelman, “The photodecontamination of cellular blood components: mechanisms and use of photosensitization in transfusion medicine,” Transfusion Medicine Reviews 10(1), 15–22 (1996).

7. D. Korneev, O. Kurskaya, K. Sharshov, J. Eastwood, and M. Strakhovskaya, “Ultrastructural Aspects of Photodynamic Inactivation of Highly Pathogenic Avian H5N8 Influenza Virus,” Viruses 11(10), 955 (2019).

8. K. Sharshov, M. Solomatina, O. Kurskaya, I. Kovalenko, E. Kholina, V. Fedorov, G. Meerovich, A. Rubin, and M. Strakhovskaya, “The Photosensitizer Octakis(cholinyl)zinc Phthalocyanine with Ability to Bind to a Model Spike Protein Leads to a Loss of SARS-CoV-2 Infectivity In Vitro When Exposed to Far-Red LED,” Viruses 13(4), 643 (2021).

9. M. G. Strakhovskaya, G. A. Meerovich, A. N. Kuskov, S. A. Gonchukov, and V. B. Loschenov, “Photoinactivation of coronaviruses: going along the optical spectrum,” Laser Physics Letters 17(9), 093001 (2020).

10. R. I. Yakubovskaya, A. D. Plyutinskaya, E. A. Plotnikova, M. A. Grin, and A. F. Mironov, “Comparative in vitro study of different classes of photosensitizers. Pyropheophorbides and chlorines,” Russian Journal of Biotherapy 14(1), 43–51 (2015) [in Russian].

11. G. A. Meerovich, E. V. Akhlyustina, I. G. Tiganova, E. A. Lukyanets, E. A. Makarova, E. R. Tolordava, O. A. Yuzhakova, I. D. Romanishkin, N. I. Philipova, Yu. S. Zhizhimova, S. A. Gonchukov, Yu. M. Romanova, and V. B. Loschenov, “Photodynamic inactivation of Pseudomonas aeruginosa bacterial biofilms using new polycationic photosensitizers,” Laser Physics Letters 16(11), 115603 (2019).

12. B. W. Pogue, L. Lilge, M. S. Patterson, B. C. Wilson, and T. Hasan, “Absorbed photodynamic dose from pulsed versus continuous wave light examined with tissue-simulating dosimeters,” Applied Optics 36(28), 7257–7269 (1997).

13. V. A. Svyatchenko, S. D. Nikonov, A. P. Mayorov, M. L. Gelfond, and V. B. Loktev, “Antiviral photodynamic therapy: Inactivation and inhibition of SARS-CoV-2 in vitro using methylene blue and Radachlorin,” Photodiagnosis and Photodynamic Therapy 33, 102112 (2021).

14. R. I. Yakubovskaya, Е. А. Plotnikova, A. D. Plyutinskaya, N. B. Morozova, V. I. Chissov, E. A. Makarova, S. V. Dudkin, E. A. Lukyanets, and G. N. Vorozhtsov, “Photophysical properties and in vitro and in vivo photoinduced antitumor activity of cationic salts of meso-tetrakis(N-alkyl-3-pyridyl)bacteriochlorins,” Journal of Photochemistry and Photobiology B: Biology 130, 109–114 (2014).

15. N. B. Morozova, R. I. Yakubovskaya, V. I. Chissov, V. M. Negrimovskiy, and O. A. Yuzhakova, “In vivo photo-induced activity of positively charged zinc phthalocyanine used for photodynamic therapy for malignancies,” Russian Journal of Oncology 17(1), 23–28 (2012) [in Russian].






© 2014-2021 Samara National Research University. All Rights Reserved.
Public Media Certificate (RUS). 12+