Study of the Diffusion of Tetracycline in the Dentin of the Human Tooth Ex Vivo

Alexey A. Selifonov (Login required)
Education and Research Institute of Nanostructures and Biosystems, Saratov State University, Russian Federation

Tatyana Yu. Rusanova
Institute of Chemistry, Saratov State University, Russian Federation

Ekaterina I. Selifonova
Education and Research Institute of Nanostructures and Biosystems, Saratov State University, Russian Federation

Valery V. Tuchin
Science Medical Center, Saratov State University, Russian Federation


Paper #3493 received 10 May 2022; revised manuscript received 11 Jul 2022; accepted for publication 18 Jul 2022; published online 15 Aug 2022.

DOI: 10.18287/JBPE22.08.030303

Abstract

Currently, manufacturers of tetracycline and tetracycline antibiotics necessarily warn about possible side effects when using tetracycline in children under 8 years of age and pregnant women. When tetracycline is used in utero or after birth, before the teeth erupt through the gum during mineralization or calcification, the antibiotic binds to calcium ions, which causes persistent gray or brown stains or often streaks on the teeth. In the present study, the diffuse reflectance spectroscopy with an integrating sphere was used to determine the molecular diffusion coefficient of tetracycline in the dentin of a human tooth ex vivo, which was found as = (6.49 ± 1.12) · 10–6 cm2/s. The dependences following from Fick’s second law and the modified Bouguer-Lambert-Beer law, as well as restrictions for the free diffusion model, were used in the calculations. It was found that when dentinal samples are saturated with tetracycline hydrochloride, the total transmittance decreases over the entire studied wavelength range from 200 to 800 nm. These studies are important for clinical practice.

Keywords

tetracycline hydrochloride; human dentin; molecular diffusion; diffuse reflectance spectroscopy; integrating sphere; free diffusion model

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References


1. R. A. Harvery, P. C. Champe (eds.), Lippincott’s Illustrated Reviews: Pharmacology, 4th ed., Lippincott, Williams & Wilkins (2009). ISBN: 978-0-781-77155-9.

2. “Tetracycline”. The American Society of Health-System Pharmacists. Archived from the original on 28 December 2016 (accessed 12.04.2022). [https://www.ashp.org/Search#q=Tetracycline&firstQueryCause=searchFromLink].

3. J. Enabulele, N. M. Chukwumah, and O. Enabulele, “Tetracycline use in children and knowledge of its oral implications among nursing mothers,” Pediatric Dental Journal 30(3), 224–230 (2020).

4. V. Madhu, R. Rajesh, K. K. R. Ealla, S. R. Velidandla, and S. Santoshi, “Tetracycline-Induced Discoloration of Deciduous Teeth: Case Series,” Journal of International Oral Health 6(3), 115–119 (2014).

5. I. S. Wallman, H. B. Hilton, “Teeth pigmented by tetracycline,” Lancet 279(7234), 827–829 (1962).

6. A. Kadam, M. Ganachari, K. B. Mahendra, and S. Gurunath, “Drug Induced Tooth Discolouration,” The Internet Journal of Dental Science 7(2), 10979 (2008).

7. A. R. Sanchez, R. S. Rogers, and P. J. Sheridan, “Tetracycline and other tetracycline-derivative staining of the teeth and oral cavity,” International Journal of Dermatology 43, 709–715 (2004).

8. H. U. Luder, L. Antonini, “Discoloration of teeth from tetracyclines - Even today?” Schweizer Monatsschrift für Zahnmedizin SMfZ 121(5), 414–431 (2011).

9. Z. Zhu, Q. Yu, G. Qi, J. Yang, Y. Ni, W. Ruan, and L. Fang, “Tigecycline-Induced Tooth Discoloration in Children Younger than Eight Years, ” Antimicrob Agents Chemother 65(9), e0085421 (2021).

10. M. L. Good, D. L. Hussey, “Minocycline: stained devil?” British Journal of Dermatology 149(2), 237–239 (2003).

11. E. Bouderlique, L. Nollet, E. Letavernier, and O. M. Vanakker, “Minocycline Counteracts Ectopic Calcification in a Murine Model of Pseudoxanthoma Elasticum: A Proof-of-Concept Study,” International Journal of Molecular Sciences 23(3), 1838–1843 (2022).

12. W. Qin, Q-Q. Wan, Y-X Ma, and C-Y Wang, “Manifestation and Mechanisms of Abnormal Mineralization in Teeth,” ACS Biomaterials Science and Engineering (2021).

13. M. S. Thomas, C. Denny, “Medication-Related Tooth Discoloration: A Review,” Clinical Dentistry 41(5), 440–445 (2014).

14. S. Tsubura, “Clinical evaluation of three months’ nightguard vital bleaching on tetracycline-stained teeth using Polanight 10% carbamide gel: 2-year follow-up study,” Odontology 98(2), 134–138 (2010).

15. A. Faheem, “Tetracycline: Classification, Structure Activity Relationship and Mechanism of Action as a Theranostic Agent for Infectious Lesions-A Mini Review,” Biomedical Journal of Scientific & Technical Research 7(2), 001475 (2018).

16. Y. He, Y.-Y. Huang, L. Xi, J. A. Gelfand, and M. R. Hamblin, “Tetracyclines function as dual-action light-activated antibiotics, ” PLoS ONE 13(5), e0196485 (2018).

17. A. V. Belikov, S. N. Smirnov, and A. D. Tavalinskaya, “Laser Delivery and Spectral Study of a Chlorine-Containing Drug for the Treatment of Onychomycosis at Sequential Laser (lambda=2810 nm) and Photodynamic (lambda=656 +/- 10 nm) Impact, ” Optics and Spectroscopy 129(7), 754–762 (2021).

18. K. N. Agwuh, A. MacGowan, “Pharmacokinetics and pharmacodynamics of the tetracyclines including glycylcyclines,” Journal of Antimicrobial Chemotherapy 58(2), 256–265 (2006).

19. A. Schuster, H. Shwachman, “The tetracyclines: applied pharmacology,” Pediatric Clinics of North America 3(2), 295–303 (1956).

20. E. A. Genina, A. N. Bashkatov, E. E. Chikina, A. B. Knyazev, O. V. Mareev, and V. V. Tuchin, “Methylene Blue mediated laser therapy of maxillary sinusitis,” Laser Physics 16(7), 1128–1133 (2006).

21. V. V. Tuchin (Ed.), Handbook of Optical Biomedical Diagnostics, Vol. 1: Light-Tissue Interaction, 2nd ed., SPIE Press, Bellingham, WA (2016). ISBN: 978-1-628-41909-2.

22. A. V. Belikov, F. I. Feldchtein, and G. B. Altshuler, “Laser Ablation of Hard Tissues,” in Handbook of Biophotonics. Vol. 2: Photonics for Health Care, 1st ed., J. Popp, V. V. Tuchin, A. Chiou, and S. Heinemann (Eds.), Wiley-VCH Verlag GmbH, 1095–1110 (2012). ISBN: 978-3-527-41048-4.

23. A. V. Belikov, G. B. Altshuler, K. V. Shatilova, V. V. Tuchin, A. V. Skrypnik, F. I. Feldchtein, A. E. Pushkareva, and I. Cernavin, “Peroxide dental bleaching via laser microchannels and tooth color measurements,” Journal of Biomedical Optics 21(12), 125001 (2016).

24. А. А. Selifonov, V. V. Tuchin, “Optical clearing and molecular diffusivity of hard and soft oral tissues,” Chapter 36 in Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging, V. V. Tuchin, Z. Dan, and E. A. Genina (Eds.), Boca Raton, CRC Press, 629–646 (2022). ISBN 978-0-367-89509-9.

25. A. A. Selifonov, O. G. Shapoval, A. N. Mikerov, and V. V. Tuchin, “Determination of the diffusion coefficient of methylene blue solutions in dentin of a human tooth using reflectance spectroscopy and their antibacterial activity during laser exposure,” Optics and Spectroscopy 126, 758–768 (2019).

26. C. T. Hanks, J. C. Fat, J. C. Wataha, and J. F. Corcoran, “Cytotoxicity and Dentin Permeability of Carbamide Peroxide and Hydrogen Peroxide Vital Bleaching Materials, in vitro, ” Journal of Dental Research 72(5), 931–938 (1993).

27. A. A. Selifonov, V. V. Tuchin, “Optical properties of human dentin when it is immersed in glucose in vitro and the kinetics of this process,” Journal of Optical Technology 87(3) 168–174 (2020).

28. N. A. Trunina, V. V. Lychagov, and V. V. Tuchin, “OCT monitoring of diffusion of water and glycerol through tooth dentine in different geometry of wetting, ” Proceedings of SPIE 7563, 75630U (2010).

29. V. V. Tuchin, G. B. Altshuler, “Dental and oral tissue optics,” Chapter 9 in Fundamentals and Applications of Biophotonics in Dentistry, Series on Biomaterials and Bioengineering, Imperial College Press, UK, 245–300 (2007).

30. A. Kotyk, K. Janacek, Cell Membrane Transport: An Interdisciplinary Approach, Plenum Press, New York (1977).

31. V. V . Tuchin, Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnostics, 3rd ed., SPIE Press, Bellingham, WA (2015). ISBN: 978-1-628-41517-9.






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