C-MEMS Derived Glassy Carbon Glucose Sensor

Naresh Mandal
Indian Institute of Technology Goa, Ponda, India

Bidhan Pramanick (Login required)
Indian Institute of Technology Goa, Ponda, India


Paper #6476 received 13 Feb 2023; revised manuscript received 20 May 2023; accepted for publication 21 May 2023; published online 1 Aug 2023

Abstract

Diabetes is one of the most severe health diseases in the world. More than 150 million people worldwide suffer from diabetes caused by an abnormal glucose concentration in their blood and tissues. The analysis of glucose levels in the body is significant, primarily due to diabetes mellitus. Therefore, accurately detecting blood glucose is crucial for diagnosing, treating, and managing diabetes. Diabetic patients can self-manage their condition by monitoring their glucose levels. The electrochemical glucose biosensor has several advantages, including recognizing glucose specifically, low price, ease of sensor manufacture, correctness, portability, and easy operation. We have developed glassy carbon electrodes (GCE) with conventional carbon-micro-electromechanical (C-MEMS) procedures for glucose sensing. SU-8 photoresist was used as the carbon precursor. The fabricated C-MEMS-derived GCE surface has been functionalized with chitosan and glucose oxidase. Glucose oxidase is a well-known enzyme for oxidizing glucose into gluconic acid and H2O2. This reaction kinetics has been recorded with increasing glucose concentration using electrochemical analysis. In summary, we have presented an electrochemical glucose biosensor in a single-step immobilization protocol of glucose oxidase on the glassy carbon electrode (GCE) surface and obtained satisfactory responses for 1 mM to 10 mM glucose solutions.

Keywords

C-MEMS; glassy carbon; biosensor; chitosan, glucose oxidase, impedance spectroscopy; electrochemical sensing; bio-functionalization

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References


1. J. E. Shaw, R. A. Sicree, and P. Z. Zimmet, “Global estimates of the preva lence of diabetes for 2010 and 2030,” Diabetes Research and Clinical Practice 87(1), 4–14 (2010).

2. P. López-Soriano, P. Noguera, M. T. Gorris, R. Puchades, Á. Maquieira, E. Marco-Noales, and M. M. López, “Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. pruni in symptomatic field samples,” PLOS ONE 12(4), e0176201 (2017).

3. S. Ferri, K. Kojima, and K. Sode, “Review of glucose oxidases and glucose dehydrogenases: A Bird's eye view of glucose sensing enzymes,” Journal of Diabetes Science and Technology 5(5), 1068–1076 (2011).

4. K.-J. Huang, L. Wang, J. Li, T. Gan, and Y.-M. Liu, “Glassy carbon electrode modified with glucose oxidase–graphene–nano-copper composite film for glucose sensing,” Measurement 46(1), 378–383 (2013).

5. J. Chung, H. So, Choi, and T. K. S. Wong, “Recent advances in noninvasive glucose monitoring,” Medical Devices: Evidence and Research 5, 45–52 (2012).

6. O. S. Khalil, “Noninvasive glucose measurement technologies: An update from 1999 to the dawn of the New Millennium,” Diabetes Technology and Therapeutics 6(5), 660–697 (2004).

7. J. Heikenfeld, A. Jajack, B. Feldman, S. W. Granger, S. Gaitonde, G. Begtrup, and B. A. Katchman, “Accessing analytes in biofluids for peripheral biochemical monitoring,” Nature Biotechnology 37, 407–419 (2019).

8. M. Gourzi, A. Rouane, R. Guelaz, M. S. Alavi, M. B. McHugh, M. Nadi, and P. Roth, “Noninvasive glycaemia blood measurements by electromagnetic sensor: Study in static and dynamic blood circulation,” Journal of Medical Engineering & Technolog 29(1), 22–26 (2005).

9. A. J. Berger, T.-W. Koo, I. Itzkan, G. Horowitz, and M. S. Feld, “Multicomponent blood analysis by near-infrared Raman spectroscopy,” Applied Optics 38(13), 2916–2926 (1999).

10. J. Kottmann, U. Grob, J. Rey, and M. Sigrist, “Mid-Infrared fiber-coupled photoacoustic sensor for biomedical applications,” Sensors 13(1), 535–549 (2013).

11. K. V. Larin, M. S. Eledrisi, M. Motamedi, and R. O. Esenaliev, “Noninvasive blood glucose monitoring with optical coherence tomography,” Diabetes Care 25(12), 2263–2267 (2002).

12. B. H. Malik, G. L. Coté, “Real-time, closed-loop dual-wavelength optical polarimetry for glucose monitoring,” Journal of Biomedical Optics 15(1), 017002 (2010).

13. X. Chen, J. Jia, and S. Dong, “Organically modified sol-gel/chitosan composite based glucose biosensor,” Electroanalysis 15(7), 608–612 (2003).

14. X. Yang, L. Hua, H. Gong, and S. N. Tan, “Covalent immobilization of an enzyme (glucose oxidase) onto a carbon sol–gel silicate composite surface as a biosensing platform,” Analytica Chimica Acta 478(1), 67–75 (2003).

15. J. Choi, K.-J. Kim, B. Kim, H. Lee, and S. Kim, “Covalent functionalization of epitaxial graphene by Azidotrimethylsilane,” The Journal of Physical Chemistry C 113(22), 9433–9435 (2009).

16. B. Pramanick, N. Mandal, D. Mondal, C. RoyChaudhuri, and S. Chakraborty, “C-MEMS derived glassy carbon electrodes-based sensitive electrochemical biosensors,” IEEE Sensors Journal 20(21), 12472–12478 (2020).

17. L. C. Clark, C. Lyons, “Electrode systems for continuous monitoring in cardiovascular surgery,” Annals of the New York Academy of Sciences 102(1), 29–45 (1962).

18. W. Zhang, Y. Du, and M. L. Wang, “Noninvasive glucose monitoring using saliva nano-biosensor,” Sensing and Bio-Sensing Research 4, 23–29 (2015).

19. H. Yao, Y. Liao, A. R. Lingley, A. Afanasiev, I. Lähdesmäki, B. P. Otis, and B. A. Parviz, “A contact lens with integrated telecommunication circuit and sensors for wireless and continuous tear glucose monitoring,” Journal of Micromechanics and Microengineering 22(7), 075007 (2012).

20. A. J. Bandodkar, W. Jia, C. Yardımcı, X. Wang, J. Ramirez, and J. Wang, “Tattoo-based noninvasive glucose monitoring: A proof-of-concept study,” Analytical Chemistry 87(1), 394–398 (2015).

21. Z. Temoçin, “Designing of a stable and selective glucose biosensor by glucose oxidase immobilization on glassy carbon electrode sensitive to H2O2 via nanofiber interface,” Journal of Applied Electrochemistry 51(2), 283–293 (2021).

22. L. Zhang, S.-M. Yuan, L.-M. Yang, Z. Fang, and G.-C. Zhao, “An enzymatic glucose biosensor based on a glassy carbon electrode modified with manganese dioxide nanowires,” Microchimica Acta 180, 627–633 (2013).

23. L. Zhang, Q. Chen, X. Han, and Q. Zhang, “MNO2 nanoparticles and Carbon Nanofibers Nanocomposites with high sensing performance toward glucose,” Journal of Cluster Science 29(6), 1089–1098 (2018).

24. C. Kaçar, B. Dalkiran, P. E. Erden, and E. Kiliç, “An amperometric hydrogen peroxide biosensor based on Co3O4 nanoparticles and multiwalled carbon nanotube modified Glassy Carbon Electrode,” Applied Surface Science 311, 139–146 (2014).

25. Q. Mei, R. Fu, Y. Ding, L. li, A. Wang, D. Duan, and D. Ye, “Electrospinning of highly dispersed ni/COO carbon nanofiber and its application in glucose electrochemical sensor,” Journal of Electroanalytical Chemistry 847, 113075 (2019).

26. B. K. Shrestha, R. Ahmad, H. M. Mousa, I.-G. Kim, J. I. Kim, M. P. Neupane, C. H. Park, and C. S. Kim, “High-performance glucose biosensor based on chitosan-glucose oxidase immobilized polypyrrole/Nafion/functionalized multiwalled carbon nanotubes bio-nanohybrid film,” Journal of Colloid and Interface Science 482, 39–47 (2016).

27. H. Guan, D. Gong, Y. Song, B. Han, and N. Zhang, “Biosensor composed of integrated glucose oxidase with liposome microreactors/chitosan nanocomposite for amperometric glucose sensing,” Colloids and Surfaces A: Physicochemical and Engineering Aspects 574, 260–267 (2019).

28. P. A. Gallay, M. D. Rubianes, F. A. Gutierrez, and G. A. Rivas, “Avidin and glucose oxidase‐non‐covalently functionalized multiwalled carbon nanotubes: A new analytical tool for building a bienzymatic glucose biosensor,” Electroanalysis 31(10), 1888–1894 (2019).

29. M. Baghayeri, “Glucose sensing by a glassy carbon electrode modified with glucose oxidase and a magnetic polymeric nanocomposite,” RSC Advances 5(24), 18267–18274 (2015).

30. Y. Yuan, Y. Wang, H. Wang, and S. Hou, “Gold nanoparticles decorated on single layer graphene applied for electrochemical ultrasensitive glucose biosensor,” Journal of Electroanalytical Chemistry 855, 113495 (2019).

31. G. Bharath, R. Madhu, S.-M. Chen, V. Veeramani, A. Balamurugan, D. Mangalaraj, C. Viswanathan, and N. Ponpandian, “Enzymatic electrochemical glucose biosensors by Mesoporous 1D hydroxyapatite-on-2d reduced graphene oxide,” Journal of Materials Chemistry B 3(7), 1360–1370 (2015).

32. Q. Zeng, J.-S. Cheng, X.-F. Liu, H.-T. Bai, and J.-H. Jiang, “Palladium nanoparticle/Chitosan-grafted graphene nanocomposites for construction of a glucose biosensor,” Biosensors and Bioelectronics 26(8), 3456–3463 (2011).

33. Y. Wang, Y. Li, L. Tang, J. Lu, and J. Li, “Application of graphene-modified electrode for selective detection of dopamine,” Electrochemistry Communications 11(4), 889–892 (2009).

34. X. Kang, J. Wang, H. Wu, I. A. Aksay, J. Liu, and Y. Lin, “Glucose oxidase–graphene–Chitosan modified electrode for direct electrochemistry and glucose sensing,” Biosensors and Bioelectronics 25(4), 901–905 (2009).

35. C. Wang, G. Jia, L. H. Taherabadi, and M. J. Madou, “A novel method for the fabrication of high-aspect ratio C-MEMS structures,” Journal of Microelectromechanical Systems 14(2), 348–358 (2005).

36. B. Pramanick, M. Vazquez-Pinon, A. Torres-Castro, S. O. Martinez-Chapaa, and M. Madou, “Effect of pyrolysis process parameters on electrical, physical, chemical and electrochemical properties of SU-8-derived carbon structures fabricated using the C-MEMS process,” Materials Today: Proceedings 5(3), 9669–9682 (2018).

37. J. Huang, “Diffusion impedance of electroactive materials, electrolytic solutions and porous electrodes: Warburg impedance and beyond,” Electrochimica Acta 281, 170–188 (2018).

38. I. Lelidis, G. Barbero, “Role of the displacement current on Warburg-type behavior,” Physical Review E 95(5), 052604 (2017).

39. J. Huang, “Generalization of porous electrode theory for Noninteger Dimensional Space,” The Journal of Physical Chemistry C 122(1), 557–565 (2018).

40. H. Li, C. Zhao, X. Wang, J. Meng, Y. Zou, S. Noreen, L. Zhao, Z. Liu, H. Ouyang, P. Tan, M. Yu, Y. Fan, Z. L. Wang, and Z. Li, “Fully bioabsorbable capacitor as an energy storage unit for Implantable Medical Electronics,” Advanced Science 6(6), 1801625 (2019).

41. M. Mazaheri, A. Simchi, and H. Aashuri, “Enzymatic biosensing by covalent conjugation of enzymes to 3D-networks of graphene nanosheets on arrays of vertically aligned gold nanorods: Application to voltammetric glucose sensing,” Microchimica Acta 185, 178 (2018).

42. N. Elgrishi, K. J. Rountree, B. D. McCarthy, E. S. Rountree, T. T. Eisenhart, and J. L. Dempsey, “A practical beginner’s guide to cyclic voltammetry,” Journal of Chemical Education 95(2), 197–206 (2018).




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