Estimating of Fraction of Weakly Deformable Erythrocytes in a Blood Sample Based on the Method of Laser Ektacytometry and a Database of Simulated Diffraction Patterns

Vladislav D. Ustinov orcid
M. V. Lomonosov Moscow State University, Russia

Evgeniy G. Tsybrov orcid (Login required)
M. V. Lomonosov Moscow State University, Russia

Sergey Y. Nikitin orcid
M. V. Lomonosov Moscow State University, Russia


Paper #7216 received 21 Feb 2023; revised manuscript received 15 Jun 2023; accepted for publication 16 Jun 2023; published online 22 Jul 2023.

Abstract

The problem of measuring the fraction of weakly deformable erythrocytes in a blood sample by the laser diffractometry in a shear flow (ektacytometry) is considered. The algorithm for measuring this parameter is proposed, based on a comparison of experimentally observed diffraction patterns with ones calculated in the bimodal ensemble approximation, when only two types of erythrocytes are present in a blood sample – normal (deformable) and rigid (non-deformable) erythrocytes. The accuracy of the algorithm was estimated by the method of numerical experiment and the area of its applicability was determined.

Keywords

erythrocyte deformability; laser ektacytometry; data processing algorithms

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References


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