C-MEMS Derived Glassy Carbon Glucose Sensor

Naresh Mandal
Indian Institute of Technology Goa, Ponda, India

Bidhan Pramanick (Login required)
Indian Institute of Technology Goa, Ponda, India


Paper #6476 received 13 Feb 2023; revised manuscript received 20 May 2023; accepted for publication 21 May 2023; published online 1 Aug 2023

Abstract

Diabetes is one of the most severe health diseases in the world. More than 150 million people worldwide suffer from diabetes caused by an abnormal glucose concentration in their blood and tissues. The analysis of glucose levels in the body is significant, primarily due to diabetes mellitus. Therefore, accurately detecting blood glucose is crucial for diagnosing, treating, and managing diabetes. Diabetic patients can self-manage their condition by monitoring their glucose levels. The electrochemical glucose biosensor has several advantages, including recognizing glucose specifically, low price, ease of sensor manufacture, correctness, portability, and easy operation. We have developed glassy carbon electrodes (GCE) with conventional carbon-micro-electromechanical (C-MEMS) procedures for glucose sensing. SU-8 photoresist was used as the carbon precursor. The fabricated C-MEMS-derived GCE surface has been functionalized with chitosan and glucose oxidase. Glucose oxidase is a well-known enzyme for oxidizing glucose into gluconic acid and H2O2. This reaction kinetics has been recorded with increasing glucose concentration using electrochemical analysis. In summary, we have presented an electrochemical glucose biosensor in a single-step immobilization protocol of glucose oxidase on the glassy carbon electrode (GCE) surface and obtained satisfactory responses for 1 mM to 10 mM glucose solutions.

Keywords

C-MEMS; glassy carbon; biosensor; chitosan, glucose oxidase, impedance spectroscopy; electrochemical sensing; bio-functionalization

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