Long-term fluorescence of erythrosine in alive mammalian cells

Valeriya S. Maryakhina orcid (Login required)
Orenburg State University, Russia


Paper #3078 received 2016.07.18 revised manuscript received 2016.09.22; accepted for publication 2016.09.29; published online 2016.09.30.

DOI: 10.18287/JBPE16.02.030303

Abstract

Investigation of triplet states of probes molecules located in cells and tissues is used for research of pathological processes included cancer development due to high sensitivity of triplet states lifetime to surround medium. In the work the long-term fluorescence of erythrosine located in alive mammalian cells has been analyzed by mathematical simulation. The model considers all basic processes of relaxation of probe triplet states in biological media. It is obtained that signal intensity and its form depends on oxygen concentration and viscosity of cells cytoplasm in which the dye diffuses. Interaction of erythrosine with the variety of extinguishers (included immobilization process into peptides molecules) does not impact on signal of the delayed fluorescence. Their concentration impact on cell viscosity mean only. The most important extinguisher is molecular oxygen impacting on signal. The correlation between theoretical and experimental results let to consider that the model is adequate. 

Keywords

long-term fluorescence; erythrosine; singlet oxygen; mammalian cells; fluorescent diagnostics.

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