Laser Illumination in Live Cell Microscopy: Scattering and Structured Illumination

Herbert Schneckenburger (Login required)
Aalen University, Institute of Applied Research, Germany

Verena Richter
Aalen University, Institute of Applied Research, Germany

Mathis Piper
Aalen University, Institute of Applied Research, Germany

Michael Wagner
Aalen University, Institute of Applied Research, Germany


Paper #3167 received 2 Mar 2017; revised manuscript received 4 Apr 2017; accepted for publication 7 Apr 2017; published online 12 Apr 2017.

DOI: 10.18287/JBPE17.03.010304

Abstract

Two types of laser illumination in live cell microscopy with a focus on the sample or in the aperture plane of the microscope objective lens are distinguished. For the second case two examples are described, namely light scattering microscopy with angular resolution and Structured Illumination Microscopy (SIM) with two interfering laser beams. Appropriate applications include morphological studies of cells undergoing apoptosis and mitochondrial imaging with increased resolution.

Keywords

Living cells; light scattering microscopy; super-resolution microscopy; structured illumination; SIM; apoptosis; mitochondrial imaging

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