Delineating nonmelanoma skin cancer margins using terahertz and optical imaging

Anna N. Yaroslavsky (Login required)
Department of Physics and Applied Physics, University of Massachusetts, Lowell, MA, USA
Department of Dermatology, Massachusetts General Hospital, Boston, MA, USA

Cecil Joseph
Department of Physics and Applied Physics, University of Massachusetts, Lowell, MA, USA

Rakesh Patel
Department of Physics and Applied Physics, University of Massachusetts, Lowell, MA, USA

Alona Muzikansky
MGH Biostatistics Center, Massachusetts General Hospital, Boston, MA, USA

Victor A. Neel
Department of Dermatology, Massachusetts General Hospital, Boston, MA, USA

Robert H. Giles
Department of Physics and Applied Physics, University of Massachusetts, Lowell, MA, USA


Paper #3170 received 8 Mar 2017; revised manuscript received 24 Mar 2017; accepted for publication 25 Mar 2017; published online 29 Mar 2017.

DOI: 10.18287/JBPE17.03.010301

Abstract

We evaluated terahertz reflectance imaging and a combination of terahertz and optical techniques for delineating nonmelanoma skin cancers. After imaging, each terahertz pixel of each specimen was classified as cancerous or normal using a previously determined threshold. Subsequently, cancerous terahertz pixels were re-evaluated with polarization-sensitive optical technique. This additional information enabled us to re-classify each cancerous terahertz pixel using morphological appearance of the structures in the optical images. If the structures in the terahertz pixel appeared normal, the pixel was considered normal. The generalized linear mixture model was implemented to determine the sensitivity and specificity of each method. The model tested the probability of each terahertz pixel being diagnosed as a match with histology. For terahertz imaging alone, the sensitivity and specificity were 82% and 94%, respectively. For multimodal imaging, the sensitivity and specificity increased to 96 % and 99%, respectively. Multimodal terahertz-optical imaging has potential for the intraoperative assessment of tumor margins.

Keywords

imaging; cancer

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References


1. M. Al-Arashi, E. Salomatina, and A. N. Yaroslavsky, “Multimodal Confocal Microscopy for the Detection of Nonmelanoma Skin Cancers,” Las. Surg. Med. 39(9), 706-15 (2007).

2. A. N. Yaroslavsky, J. Barbosa, V. Neel, C. DiMarzio, and R. R. Anderson, “Combining multi-spectral polarized-light imaging and confocal microscopy for localization of nonmelanoma skin cancer,” J Biomed Opt 10(1), 014011 (2005).

3. T. Gambichler, A. Orlikov, R. Vasa, G. Moussa, K. Hoffmann, M. Stücker, P. Altmeyer, and F. G. Bechara, “In vivo optical coherence tomography of basal cell carcinoma,” J Dermatol Sci 45(3), 167-173 (2007).

4. J. Welzel, “Optical coherence tomography in dermatology: a review,” Skin Res Tech 7(1), 1-9 (2001).

5. S. L. Jacques, J. R. Roman, and K. Lee, “Imaging superficial tissues with polarized light,” Las. Surg. Med. 26(2), 119-129 (2000).

6. A. N. Yaroslavsky, V. A. Neel, and R. R Anderson, “Demarcation of nonmelanoma skin cancer margins in thick excisions using multispectral polarized light imaging,” J Inv. Derm. 121(2), 259-266 (2003).

7. A. N. Yaroslavsky, V. Neel, and R. R. Anderson, “Fluorescence polarization imaging for delineating nonmelanoma skin cancers,” Opt. Lett. 29(17), 2010-2012 (2004).

8. A. N. Yaroslavsky, E. V. Salomatina, V. Neel, R. Anderson, and T. Flotte, “Fluorescence polarization of tetracycline derivatives as a technique for mapping nonmelanoma skin cancers,” J Bio. Opt. 12(1), 014005 (2007).

9. Z. Tannous, M. Al-Arashi, S. Shah, and A. N. Yaroslavsky, “Delineating Melanoma Using Multimodal Polarized Light Imaging,” Las. Surg. Med. 41(1), 10-16 (2009).

10. C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Las. Surg. Med. 40(7), 461-467 (2008).

11. J. Choi, J. Choo, H. Chung, D. G. Gweon, J. Park, H. J. Kim, S. Park, and C. H. Oh, “Direct observation of spectral differences between normal and basal cell carcinoma (BCC) tissues using confocal Raman microscopy,” Biopolymers 77(5), 264-272 (2005).

12. R. M. Woodward, B. E. Cole, V. P. Wallace, R. J. Pye, D. D. Arnone, E. H. Linfield, and M. Pepper, “Terahertz pulse imaging in reflection geometry of human skin cancer and skin tissue,” Phys. Med. Bio. 47(21), 3853-3863 (2002).

13. E. Pickwell, B. E. Cole, A. J. Fitzgerald, M. Pepper, and V. P. Wallace, “In vivo study of human skin using pulsed terahertz radiation,” Phys. Med. Bio. 49(9), 1595-1607 (2004).

14. V. P. Wallace, A. J. Fitzgerald, E. Pickwell, R. J. Pye, P. F. Taday, N. Flanagan, and T. Ha, “Terahertz pulsed spectroscopy of human basal cell carcinoma,” App. Spectr. 60(10), 1127-1133 (2006).

15. B. Fan, V. A. Neel, A. N. and Yaroslavsky, “Multimodal Imaging for Nonmelanoma Skin Cancer Margin Delineation,” Las. Surg. Med., to be published (2016).

16. C. S. Joseph, R. Patel, V. Neel, R. H. Giles, and A. N. Yaroslavsky, “Imaging of ex vivo nonmelanoma skin cancers in the optical and terahertz spectral regions,” J. Biophoton. 7(5), 295-303 (2012).

17. E. Salomatina, A. Muzikansky, V. Neel, and A. N. Yaroslavsky, “Multimodal optical imaging and spectroscopy for the intraoperative mapping of nonmelanoma skin cancer,” J Appl Phys 105(10), 102010-102017 (2009).




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