Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues

Tsanislava Genova; Ekaterina Borisova; Oksana Semyachkina-Glushkovskaya; Dmitry Gorin; Daniil Bratashov; Ivan Terziev

doi:10.18287/JBPE17.03.040305

Authors

Tsanislava Genova Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria
Ekaterina Borisova Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria Saratov State University, Russian Federation
Oksana Semyachkina-Glushkovskaya Saratov State University, Russian Federation
Dmitry Gorin Saratov State University, Russian Federation
Daniil Bratashov Saratov State University, Russian Federation
Ivan Terziev University Hospital “Tsaritsa Yoanna”-ISUL, Sofia, Bulgaria

DOI:

https://doi.org/10.18287/JBPE17.03.040305

Keywords:

Autofluorescence, gastrointestinal tissues, colon carcinoma, confocal fluorescent microscopy (CFM)

Abstract

New more sensitive diagnostic modalities, for either analysis of biopsy tissue samples or on site, in vivo microscopy tissue examination, emerge to aid the detection and accurate diagnosis of gastrointestinal cancer. One of the most intensively investigated techniques is the fluorescence spectroscopy approach.

We present our investigation of the detailed macro and micro fluorescence characteristics of ex-vivo fresh and fixed tissue samples. The fluorescence spectra of unprocessed cancerous and healthy gastrointestinal (GIT) tissue, obtained with two approaches fluorescence and synchronous fluorescence spectroscopy were compared with microspectroscopy performed with laser scanning confocal fluorescence microscopy (LSCFM) system in Lambda-scan regime over fixed tissue samples.

The evaluated optical macro- and micro- spectroscopy characteristics are presented and disscused in order to improve the current understanding of the origin of autofluorescence contrast between healthy and cancerous tissues and its application for improvement of the existing diagnostic modalities.

Author Biographies

Tsanislava Genova, Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria

PhD student at Laboratory "Biophotonics"
Ivan Terziev, University Hospital “Tsaritsa Yoanna”-ISUL, Sofia, Bulgaria

Dr. Ivan Terziev is Head of department of Pathology and and Senior Assistant at University Hospital “Tsaritsa Yoanna”-ISUL, Sofia, Bulgaria

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