Fluorescent diagnostics of benign breast diseases and breast cancer

Valeriya S. Maryakhina orcid (Login required)
Kumertau branch of Orenburg State University, Kumertau, Russia

Yulia S. Korneva
Smolensk State Medical University, Russia
Smolensk Regional Institute of Pathology, Russia

Igor V. Chekurov
Orenburg State Agriculture University, Russia

Oksana A. Shisterova
Smolensk Oncological Dispensary, Russia


Paper #3271 received 1 Dec 2017; revised manuscript received 19 Dec 2017; accepted for publication 20 Dec 2017; published online 31 Dec 2017. [Saratov Fall Meeting 2017 Special Issue].

DOI: 10.18287/JBPE17.03.040306

Abstract

In the study the spectra of fluorescence excitation and autofluorescence of histological sections with different pathological processes in breast were measured. Additionally, element composition of the tissues was investigated by scanning electron microscopy. It has been shown that spectra of fluorescence excitation of benign breast diseases (fibroadenoma and fibrocystic breast disease) have low maximum at 232 nm and high maximum at 260 nm. Also, fluorescence spectra of histological samples change during tumor growth and have two maxima (335 and 420 nm). The registered spectra are result of emission of such fluorophores as fatty acids, tryptophan, NADH, vitamin B6, collagen and bilirubin. The obtained spectral data correlate well with data of element analysis. Selenium status of the tissues was investigated by scanning electronic microscope. High concentration if selenium was detected only in fibroadenoma. In breast cancer samples and fibrocystic breast disease low selenium content was detected. It may confirm higher risk for malignant transformation of fibrocystic breast disease than fibroadenoma. Our results may be helpful for cancer diagnostics and for prognosis prediction.

Keywords

breast cancer; benign breast disease; fluorescence; optical biomedical diagnostics

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References


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