Registration of Regeneration in Planarians from Photographic Images

Kharlampiy Tiras (Login required)
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Obl., Russian Federation
Pushchino State Institute of Natural Science, Pushchino, Moscow Obl., Russian Federation

Leonid Mestetsky
Lomonosov Moscow State University, Russian Federation
Federal Research Centrе “Computer Science and Control” of the Russian Academy of Sciences, Moscow, Russian Federation

Svetlana Nefedova
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Obl., Russian Federation
Pushchino State Institute of Natural Science, Pushchino, Moscow Obl., Russian Federation

Nikita Lomov
Federal Research Centrе “Computer Science and Control” of the Russian Academy of Sciences, Moscow, Russian Federation


Paper #3430 received 25 May 2021; revised manuscript received 6 Sep 2021; accepted for publication 6 Sep 2021; published online 30 Sep 2021.

DOI: 10.18287/JBPE21.07.030303

Abstract

In this study, an approach to constructing a mathematical model for quantifying the dynamics of regeneration of planarian flatworms in biological experiments is considered, based on an analysis of a series of digital microscopic images. A method is proposed to describe the body shape of a planarian using a continuous morphological model, based on the concept of a medial representation of the worm’s silhouette. The silhouette in this case is a polygon approximating the contours of the planarian’s body. The medial representation of the figure includes a medial axis and a radial function that describes the width of the figure relative to the medial axis. We propose a set of morphological criteria for assessing the dynamics of regeneration based on a continuous morphological model and present the results of computational experiments.

Keywords

planarians; digital morphometry; medial representation; image skeleton

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References


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