Registration of Regeneration in Planarians from Photographic Images

Kharlampiy Tiras; Leonid Mestetsky; Svetlana Nefedova; Nikita Lomov

doi:10.18287/JBPE21.07.030303

Authors

Kharlampiy Tiras Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Obl., Russian Federation Pushchino State Institute of Natural Science, Pushchino, Moscow Obl., Russian Federation
Leonid Mestetsky Lomonosov Moscow State University, Russian Federation Federal Research Centrе “Computer Science and Control” of the Russian Academy of Sciences, Moscow, Russian Federation
Svetlana Nefedova Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Moscow Obl., Russian Federation Pushchino State Institute of Natural Science, Pushchino, Moscow Obl., Russian Federation
Nikita Lomov Federal Research Centrе “Computer Science and Control” of the Russian Academy of Sciences, Moscow, Russian Federation

DOI:

https://doi.org/10.18287/JBPE21.07.030303

Keywords:

planarians, digital morphometry, medial representation, image skeleton

Abstract

In this study, an approach to constructing a mathematical model for quantifying the dynamics of regeneration of planarian flatworms in biological experiments is considered, based on an analysis of a series of digital microscopic images. A method is proposed to describe the body shape of a planarian using a continuous morphological model, based on the concept of a medial representation of the worm’s silhouette. The silhouette in this case is a polygon approximating the contours of the planarian’s body. The medial representation of the figure includes a medial axis and a radial function that describes the width of the figure relative to the medial axis. We propose a set of morphological criteria for assessing the dynamics of regeneration based on a continuous morphological model and present the results of computational experiments.

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