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In Vitro Study of Tetracycline Penetration into the Enamel and Dentin of the Tooth by Fluorescence Spectroscopy
Alexey A. Selifonov
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Saratov State University, Russian Federation
Paper #9015 received 2 Sep 2023; revised manuscript received 18 Oct 2023; accepted for publication 18 Oct 2023; published online 10 Dec 2023.
DOI: 10.18287/JBPE23.09.040309
Abstract
With the use of tetracycline in childhood, as well as with intrauterine or ectopic exposure before teething through the gums during mineralization or calcification of the teeth, the antibiotic binds to calcium ions in the teeth. This results in permanent staining of the teeth. In dentistry, the term “tetracycline teeth” even arose. During teething and exposure to light, calcium-bound tetracycline oxidizes, causing the enamel to change color from fluorescent yellow to brown. In this study, using fluorescence spectroscopy, it was found that tetracycline exhibits characteristic fluorescence peaks in the dentin and in the enamel of the teeth after exposure of the antibiotic solution to the extracted teeth in vitro. Thus, it was shown that in non-carious fully mineralized human dental tissues, tetracycline penetrates quite well into the dentin matrix and, to a much lesser extent, into the enamel matrix, retaining its fluorescence.
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1. V. V. Tuchin (Ed.), Handbook of Optical Biomedical Diagnostics, Vol. 1: Light-Tissue Interaction, 2nd ed., SPIE Press, Bellingham, WA (2016). ISBN: 978-1-628-41909-2.
2. V. V. Tuchin, G. B. Altshuler, “Dental and oral tissue optics,” Chapter 9 in Fundamentals and Applications of Biophotonics in Dentistry, Series on Biomaterials and Bioengineering, Imperial College Press, UK, 245–300 (2007).
3. J. J. Ten Bosch, J. C. Coops, “Tooth color and reflectance as related to light scattering and enamel hardness,” Journal of Dental Research 74(1), 374–380(1995).
4. C. Klein, T. Connert, C. Ohle, and С. Meller, “How well can today’s tooth colored dental restorative materials reproduce the autofluorescence of human teeth? – Ambition and reality!” Journal of Esthetic and Restorative Dentistry 33(5), 720–738 (2021).
5. C. C. Ko, D. H. Yi, D. J. Lee, J. Kwon, F. Garcia-Godoy, and Y. H. Kwon, “Diagnosis and staging of caries using spectral factors derived from the blue laser-induced autofluorescence spectrum,” Journal of Dental Research 67, 77–83 (2017).
6. S. H. Oh, J. Y. Choi, and S. H. Kim,“Evaluation of dental caries detection with quantitative light-induced fluorescence in comparison to different field of view devices,” Scientific Reports12, 6139 (2022).
7. С. Longbottom, В. Vernon, Е. Perfect, А. М. Haughey, А. Christie, and N. Pitts, “Initial investigations of a novel bioluminescence method for imaging dental demineralization,” Clinical and Experimental Dental Research 7(5), 786–794 (2021).
8. J. W. Kim, J. K. Park, F. Garcia-Godoy, and Y. H. Kwon, “Evaluation of autofluorescence and image of oral pathogens and the tooth,” American Journal of Dentistry 35(4), 212–216 (2022).
9. А. Garcez, L. C. Barros, М. Fernandes, and R. Nepomuceno, “Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews,” Photodiagnosis and Photodynamic Therapy 30, 101707 (2020).
10. J. Y. Lee, H. I. Jung, and B. I. Kim, “A novel model to predict tooth bleaching efficacy using autofluorescence of the tooth,” Journal of Dentistry 116, 103892 (2022).
11. С. V. Cioban, S. А. Petrutiu, D. С. Condor, and W. А. Uriciuc, “The use of autofluorescence for screening and early detection of oral potentially malignant disorders – A narrative review,” Romanian Journal of Stomatology 68(4), 153–159 (2022).
12. M. Piriou, C. Lorenzo, I. Raymond-Letron, S. Coronas-Dupuis, L. Pieruccioni, J. Rouquette, C. Guissard, J. Chaumont, L. Casteilla, V. Planat-Benard, P. Kemoun, and P. Monsarrat, “A Spectral Principal Component Analysis-Based Framework for Composite Hard/Soft Tissue Fluorescence Image Investigation,” Frontiers in Physiology 13, 899626 (2022).
13. Y.-K. Lee, “Opalescence of human teeth and dental esthetic restorative materials,” Dental Materials Journal 35(6), 845–854 (2016).
14. M. S. Cho, B. Yu, and Y. K. Lee, “Opalescence of all-ceramic core and veneer materials,” Dental Materials 25(6), 695–702 (2009).
15. C. A. M. Volpato, M. R. Da Cunha Pereira, and F. S. Silva, “Fluorescence of natural teeth and restorative materials, methods for analysis and quantification: A literature review, ”Journal of Esthetic and Restorative Dentistry 30(5), 397–407 (2018).
16. С. Klein, С. Meller, D. Wolff, and С. Ohle, “The fluorescence of resin-based composites: An analysis after ten years of aging,” Dental Materials Journal 40(1), 94–100 (2021).
17. J. Enabulele, N. M. Chukwumah, and O. Enabulele, “Tetracycline use in children and knowledge of its oral implications among nursing mothers,” Pediatric Dental Journal 30(3), 224–230 (2020).
18. I. Tlili, G. Caria, B. Ouddane, I. Ghorbel-Abid, R. Ternane, M. Trabelsi-Ayadi, and S. Net, “Simultaneous detection of antibiotics and other drug residues in the dissolved and particulate phases of water by an off-line SPE combined with on-line SPE-LC-MS/MS: Method development and application,” Science of the Total Environment 563–564, 424–433 (2016).
19. W. Cheng, L. Jiang, N. Lu, L. Ma, X. Sun, Y. Luo, K. Lin, and C. Cui, “Development of a method for trace level determination of antibiotics in drinking water sources by high performance liquid chromatography-tandem mass spectrometry,”Analytical Methods 7(5), 1777–1787 (2015).
20. L. Goncharova, N. Kobylinska, M. E. Diaz-Garcia, and V. N. Zaitsev, “Solid-Phase Luminescence Determination of Tetracycline in Bottled Water Using Chemically Modified Silica,” Journal of Analytical Chemistry 72, 724–733 (2017).
21. Q. Liu, D. Ning, W. J. Li, Х. М. Du, Q. Wang, Y. Li, and W. Ruan, “Metal-organic framework-based fluorescent sensing of tetracycline-type antibiotics applicable to environmental and food analysis,” The Analyst 144(6), 1916–1922 (2019).
22. А. А. Oladipo, D. S. Oskouei, and М. Gazi, “Metal-organic framework-based nanomaterials as opto-electrochemical sensors for the detection of antibiotics and hormones: A review,” Beilstein Journal of Nanotechnology 14(1), 631–673 (2023).
23. Т. Wiwasuku, А. Chuaephon, Т. Puangmali, J. Boonmak, S. Ittisanronnachai, V. Promarak, and S. Youngme, “Multifunctional fluorescent Eu-MOF probe for tetracycline antibiotics and dihydrogen phosphate sensing and visualizing latent fingerprints,” RSC Advances 13, 10384–10396 (2023).
24. Y. Zhan, J. J. Tsai, and Y. C. Chen, “Zinc Ion-Based Switch-on Fluorescence-Sensing Probes for the Detection of Tetracycline,” Molecules 27(23), 8403 (2022).
25. J. Zhang, Z. Bao, J. Y. Qian, Н. Zhou, and К. Zhang, “Copper doped zinc sulfide quantum dots as ratiometric fluorescent probes for rapid and specific detection of tetracycline residues in milk,” Analytica Chimica Acta 1216, 339991 (2022).
26. D. Ravindra, G. Huang, K. Hallett, D. P. Burgner, A. Gwee, and M. J. Silva, “Antibiotic Exposure and Dental Health: A Systematic Review,” Pediatrics 152(1), e2023061350 (2023).
27. А. Schuster, Н. Shwachman, “Tetracyclines; applied pharmacology,” Pediatric Clinics of North America, 295–303 (1956).
28. A. A. Selifonov, T. Yu. Rusanova, E. I. Selifonova, and V. V. Tuchin, “Study of the Diffusion of Tetracycline in the Dentin of the Human Tooth Ex Vivo,” Journal of Biomedical Photonics & Engineering 8(3), 030303 (2022).
29. А. Kienle, R. Michels, and R. Hibst, “Magnification-a New Look at a Long-known Optical Property of Dentin,” Journal of Dental Research 85(10), 955–961 (2006).
30. Y. Shimada, K. Matin, M. Ikeda, А. Sadr, Y. Sumi, and J. Tagami, “Monitoring of cariogenic demineralization at the enamel-composite interface u sing swept-source optical coherence tomography,” Dental Materials 32(9), 1103–1112 (2016).
31. M. Villarroel, N. Fahl, A. M. De Sousa, and O. B. De Oliveira Jr., “Direct esthetic restorations based on translucency and opacity of composite resins, ” Journal of Esthetic and Restorative Dentistry 23(2), 73–87 (2011).
32. A. V. Belikov, G. B. Altshuler, K. V. Shatilova, V. V. Tuchin, A. V. Skrypnik, F. I. Feldchtein, A. E. Pushkareva, I. Cernavin, and V. Tuchin, “Peroxide dental bleaching via laser microchannels and tooth color measurements,” Journal of Biomedical Optics 21(12), 125001 (2016).
33. S. N. Siddiqui, “Dentin - Anatomy and Histolog,” Ivory Graft (accessed 18 October 2023). [https://ivorygraft.com/article/dentin-anatomy-and-histology/].
34. M. Goldberg, A. B. Kulkarni, M. Young, and A. Boskey, “Dentin: structure, composition and mineralization,” Frontiers in Bioscience (Elite edition) 3, 711–735 (2011).
35. R. P. Samusev, S. V. Dmitrienko, and A. I. Krajushkin, Basics of Clinical Dental Morphology, Russian Edition, Book on Demand Ltd. (2018). ISBN: 978-5519582513.
36. H. C. Ruschel, O. Chevitarese, “Density and diameter of dentinal tubules of first and second primary human molars--comparative scanning electron microscopy study, ” Journal of Clinical Pediatric Dentistry 26(3), 297–304 (2002).
37. I. Carneiro, S. Carvalho, R. Henrique, А. Selifonov, L. Oliveira, and V. V. Tuchin, “Enhanced Ultraviolet Spectroscopy by Optical Clearing for Biomedical Applications,” IEEE Journal of Selected Topics in Quantum Electronics 27(4), 9153126 (2021).
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