Inactivation of Bacteria in Biofilms by Shock Waves

Sergey Letuta orcid
Orenburg State University, Russian Federation

Azamat Ishemgulov orcid (Login required)
Orenburg State University, Russian Federation

Olga Davydova
Orenburg State University, Russian Federation

Maxim Grigoriev
Orenburg State University, Russian Federation


Paper #9172 received 3 Oct 2024; revised manuscript received 18 Nov 2024; accepted for publication 18 Nov 2024; published online 3 Dec 2024.

DOI: 10.18287/JBPE24.10.040311

Abstract

The processes of destruction of biofilms and inactivation of bacteria under the influence of shock acoustic waves and photodynamic treatment were studied. Shock waves were generated by locally heating the medium with nanosecond pulses of focused laser radiation. The radiation energy was converted into heat in the process of nonradiative relaxation of the excited states of organic dye molecules dissolved in the medium. The processes of biofilm removal and destruction of the extracellular matrix by shock waves are discussed. The low efficiency of inactivation of bacteria in biofilms by shock acoustic waves with peak pressure commensurate with the threshold of tensile strength of the cell wall material has been experimentally proven.

Keywords

bacterial films; shock waves; inactivation of microorganisms

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References


1. S. Sharma, J. Mohler, S. D. Mahajan, S. A. Schwartz, L. Bruggemann, and R. Aalinkeel, “Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment,” Microorganisms 11(6), 1614 (2023).

2. A. Zhao, J. Sun, and Y. Liu, “Understanding bacterial biofilms: From definition to treatment strategies,” Frontiers in Cellular and Infection Microbiology 13, 1137947 (2023).

3. A. G. Abdelhamid, A. E. Yousef, “Combating Bacterial Biofilms: Current and Emerging Antibiofilm Strategies for Treating Persistent Infections,” Antibiotics 12(6), 1005 (2023).

4. D. Liu, Q. Huang, W. Gu, and X. Zeng, “A review of bacterial biofilm control by physical strategies,” Critical Reviews in Food Science and Nutrition 62(13), 3453–3470 (2022).

5. J. Khan, S. M. Tarar, I. Gul, U. Nawaz, and M. Arshad, “Challenges of antibiotic resistance biofilms and potential combating strategies: a review,” 3 Biotech 11(4), 169 (2021).

6. J. P. Sahoo, A. P. Mishra, K. C. Samal, and A. K. Dash, “Insights into the antibiotic resistance in Biofilms – A Review,” Environment Conservation Journal 22(3), 59–67 (2021).

7. S. P. Songca, Y. Adjei, “Applications of Antimicrobial Photodynamic Therapy against Bacterial Biofilms,” International Journal of Molecular Sciences 23(6), 3209 (2022).

8. S. N. Letuta, S. N. Pashkevich, A. T. Ishemgulov, and A. N. Nikiyan, “Inactivation of Planktonic Microorganisms by Acoustic Shock Waves,” Russian Journal of Physical Chemistry A 95(4), 848–854 (2021).

9. S. N. Letuta, A. T. Ishemgulov, A. N. Nikiyan, D. A. Razdobreev, L. V. Galaktionova, D. V. Dorofeev, and D. E. Tsurko, “Mechanisms of Damage in Salmonella typhimurium and Staphylococcus aureus upon Pulse Photoexcitation of Molecular Sensitizers,” Biophysics 67(3), 419–426 (2022).

10. H. S. Yadav, D. S. Murty, S. N. Verma, K. H. C. Sinha, B. M. Gupta, and D. Chand, “Measurement of refractive index of water under high dynamic pressures,” Journal of Applied Physics 44, 2197–2200 (1973).

11. H. H. Tuson, G. K. Auer, L. D. Renner, M. Hasebe, C. Tropini, M. Salick, W. C. Crone, A. Gopinathan, K. C. Huang, and D. B. Weibel, “Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity,” Molecular Microbiology 84(5), 874–891 (2012).

12. K. K. Rohatgi-Mukherjee, Fundamentals of Photochemistry, Wiley Eastern Ltd., New Delhi, Bangalore, Bombay (1978). ISBN: 9780470265475.

13. L. M. Tokubo, P. L. Rosalen, J. De Cássia Orlandi Sardi, I. A. Freires, M. Fujimaki, J. E. Umeda, P. M. Barbosa, G. O. Tecchio, N. Hioka, C. F. De Freitas, and R. S. Suga Terada, “Antimicrobial effect of photodynamic therapy using erythrosine/methylene blue combination on Streptococcus mutans biofilm,” Photodiagnosis and Photodynamic Therapy 23, 94–98 (2018).

14. C. Fracalossi, J. Y. Nagata, D. S. Pellosi, R. S. S. Terada, N. Hioka, M. L. Baesso, F. Sato, P. L. Rosalen, W. Caetano, and M. Fujimaki, “Singlet oxygen production by combining erythrosine and halogen light for photodynamic inactivation of Streptococcus mutans,” Photodiagnosis and Photodynamic Therapy 15, 127–132 (2016).

15. S. Tobita, Y. Kaisu, H. Kobayashi, and I. Tanaka, “Study of higher excited singlet states of zinc(II)-tetraphenylporphin,” The Journal of Chemical Physics 81(7), 2962−2969 (1984).

16. H.-B. Lin, M .R. Topp, “Low quantum-yield molecular fluorescence. Aromatic hydrocarbons in solution at 300 K,” Chemical Physics Letters 48(2), 251−255 (1977).

17. D. P. Gnanadhas, M. Elango, S. Janardhanraj, C. S. Srinandan, A. Datey, R. A. Strugnell, J. Gopalan, and D. Chakravortty, “Successful treatment of biofilm infection using shock waves combined with antibiotic therapy,” Scientific Reports 5, 17440 (2015).

18. B. W. Peterson, Y. He, Y. Ren, A. Zerdoum, M. R. Libera, P. K. Sharma, A.-J. Van Winkelhoff, D. Neut, P. Stoodley, H. C. Van Der Mei, and H. J. Busscher, “Viscoelasticity of biofilms and their recalcitrance to mechanical and chemical challenges,” FEMS Microbiology Reviews 39, 234–245 (2015).

19. S. Lee, A. G. Doukas, “Laser-generated stress waves and their effects on the cell membrane,” IEEE Journal of Selected Topics in Quantum Electronics 5(4), 997–1003 (1999).

20. N. S. Soukos, S. E. Mulholland, S. S. Socransky, and A. G. Doukas, “Photodestruction of human dental plaque bacteria: enhancement of the photodynamic effect by photomechanical waves in an oral biofilm model,” Lasers in Surgery and Medicine 33(3), 161–168 (2003).

21. P. E. Huber, P. Pesterer, “In vitro and in vivo transfection of plasmid DNA in the Dunning prostate tumor R3327-AT1 is enhanced by focused ultrasound,” Gene Therapy 7, 1516−1525 (2000).

22. S. Wood, D. Metcalf, D. Devine, and C. Robinson, “Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms,” Journal of Antimicrobial Chemotherapy 57(4), 680–684 (2006).

23. J. Ghorbani, D. Rahban, S. Aghamiri, A. Teymouri, and A. Bahador, “Photosensitizers in antibacterial photodynamic therapy: an overview,” Laser Therapy 27(4), 293–302 (2018).

24. A. K. Surur, A. B. De Oliveira, S. R. De Annunzio, T. M. Ferrisse, and C. R. Fontana, “Bacterial resistance to antimicrobial photodynamic therapy: A critical update,” Journal of Photochemistry and Photobiology B: Biology 255, 112905 (2024).

25. A. Milstrey, S. Rosslenbroich, J. Everding, M. J. Raschke, R. G. Richards, T. F. Moriarty, and J. Puetzler, “Antibiofilm efficacy of focused high-energy extracorporeal shockwaves and antibiotics in vitro,” Bone & Joint Research 10(1), 77–84 (2021).

26. S. Gu, Y. Zhang, and Y. Wu, “Effects of sound exposure on the growth and intracellular macromolecular synthesis of E. coli k-12,” PeerJ 4, e1920 (2016).

27. Y. Chisti, “Sonobioreactors: Using ultrasound for enhanced microbial productivity,” Trends in Biotechnology 21(2), 89–93 (2003).

28. L. Gerdesmeyer, C. Von Eiff, C. Horn, M. Henne, M. Roessner, P. Diehl, and H. Gollwitzer, Antibacterial effects of extracorporeal shock waves,” Ultrasound in Medicine & Biology 31(1), 115–119 (2005).




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