Hide
Show all
Experimentally testing the role of blood vessels in the full scattering profile: solid phantom measurements
Dror Fixler
(Login required)
Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Israel
Paper #3096 received 2016.09.15; revised manuscript received 2016.10.28; accepted for publication 2016.10.31; published online 2016.12.23.
DOI: 10.18287/JBPE16.02.040301
Abstract
Optical methods for biomedical purposes mostly use reflected light, while few of them use the transmitted light. The blood vessels pose a challenge to these methods due to their high absorption and scattering coefficients as well as their change in size during respiration, and they are also naturally distributed in size within the body and between individuals. We suggest the full scattering profile (FSP) method in order to investigate the light at every possible exit angle. Our model of FSP successfully describes the role of the blood vessel diameter in light-tissue interaction. By means of the new point of view of FSP, we found the isobaric point, which is non-dependent on the optical properties. The uniqueness of the isobaric point is that it overcomes the shielding effect, which has known influence on the reflected light, for various vascular diameters of the same volume. We present these findings experimentally by measuring cylindrical phantoms with blood vessels in different diameters, and compare the results to our simulation results. The importance of the immunity to the shielding effect is that it allows self-calibration in clinical measurements and decreases the calibration error. In addition, by using the isobaric point we can cope with changes in blood vessel diameters and not assume microcirculation only.
Keywords
Full Text:
PDFReferences
1. S. L. Jacques, “Optical properties of biological tissues: a review,” Physics in medicine and biology 58(11), R37 (2013).
2. B. Xiong, L. Peng, X. Cao, Y. He, and E. S. Yeung, “Optical analysis of biological hydrogen sulphide: an overview of recent advancements,” Analyst 140(6), 1763-1771 (2015). Crossref
3. D. I. Ellis, D. P. Cowcher, L. Ashton, S. O’Hagan, and R. Goodacre, “Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool,” Analyst 138(14), 3871-3884 (2013).
4. R. Ankri, H. Taitelbaum, and D. Fixler, “Reflected light intensity profile of two-layer tissues: phantom experiments,” Journal of biomedical optics 16(8), 085001, (2011). Crossref
5. D. Fixler, and R. Ankri, “Subcutaneous gold nanorods detection with diffusion reflection measurement,” Journal of biomedical optics 18(6), 061226 (2013). Crossref
6. R. A. J. Groenhuis, J. J. Ten Bosch, and H. A. Ferwerda, “Scattering and absorption of turbid materials determined from reflection measurements. 2: Measuring method and calibration,” Applied optics 22(16), 2463-2467 (1983). Crossref
7. D. Jakubowski, F. Bevilacqua, S. Merritt, A. Cerussi, and B. J. Tromberg, “Quantitative absorption and scattering spectra in thick tissues using broadband diffuse optical spectroscopy,” Chapter 12 in Biomedical Optical Imaging, J. G. Fujimoto, and D. L. Farkas (eds.), Oxford University Press, New York, 330-355 (2009).
8. T. H. Pham, O. Coquoz, J. B. Fishkin, E. Anderson, and B. J. Tromberg, “Broad bandwidth frequency domain instrument for quantitative tissue optical spectroscopy,” Review of Scientific Instruments 71(6), 2500-2513, (2000). Crossref
9. M. Friebel, J. Helfmann, U. Netz, and M. Meinke, “Influence of oxygen saturation on the optical scattering properties of human red blood cells in the spectral range 250to2000nm,” Journal of biomedical optics 14(3), 034001 (2009). Crossref
10. M. Friebel, A. Roggan, G. Müller, and M. Meinke, “Determination of optical properties of human blood in the spectral range 250to1100nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions,” Journal of biomedical optics 11(3), 034021 (2006). Crossref
11. L. Zhang, A. Shi, and H. Lu, “Determination of optical coefficients of biological tissue from a single integrating-sphere,” Journal of Modern Optics 59(2), 121-125 (2012). Crossref
12. I. Feder, H. Duadi, and D. Fixler, “Experimental system of the full scattering profile of circular phantoms,” Biomed. Opt. Express 6(8), 2877-2886 (2015). Crossref
13. H. Duadi, I. Feder, and D. Fixler, “Linear dependency of full scattering profile isobaric point on tissue diameter,” Journal of biomedical optics 19(2), 026007 (2014). Crossref
14. H. Duadi, D. Fixler, and R. Popovtzer, “Dependence of light scattering profile in tissue on blood vessel diameter and distribution: a computer simulation study,” Journal of biomedical optics 18(11), 111408 (2013). Crossref
15. H. Duadi, and D. Fixler, “Influence of multiple scattering and absorption on the full scattering profile and the isobaric point in tissue,” Journal of biomedical optics 20(5), 056010 (2015). Crossref
16. I. Feder, H. Duadi, T. Dreifuss, and D. Fixler, “The influence in the full scattering profile from cylindrical tissues following changes in vessels diameter: experimental evidence for the shielding effect,” Journal of biophotonics 9(10), 1001-1008 (2015). Crossref
17. L. O. Svaasand, E. J. Fiskerstrand, G. Kopstad, L. T. Norvang, E. K. Svaasand, J. S. Nelson, and M. W. Berns, “Therapeutic response during pulsed laser treatment of port-wine stains: dependence on vessel diameter and depth in dermis," Lasers in Medical Science 10(4), 235-243 (1995).” Crossref
18. H. Liu, B. Chance, A. H. Hielscher, S. L. Jacques, and F. K. Tittel, “Influence of blood vessels on the measurement of hemoglobin oxygenation as determined by time-resolved reflectance spectroscopy,” Medical physics 22(8), 1209-1217 (1995). Crossref
19. S. L. Jacques, “Optical assessment of cutaneous blood volume depends on the vessel size distribution: a computer simulation study,” Journal of biophotonics 3(1-2), 75-81 (2010).
20. W. Verkruysse, G. W. Lucassen, J. F. de Boer, D. J. Smithies, J. S. Nelson, and M. J. C. van Gemert, “Modelling light distributions of homogeneous versus discrete absorbers in light irradiated turbid media,” Physics in Medicine and Biology 42(1), 51-65 (1997). Crossref
21. M. Firbank, E. Okada, and D. T. Delpy, “Investigation of the effect of discrete absorbers upon the measurement of blood volume with near-infrared spectroscopy,” Physics in medicine and biology 42(3), 465-477 (1997).
22. A. Talsma, B. Chance, and R. Graaff, “Corrections for inhomogeneities in biological tissue caused by blood vessels,” J. Opt. Soc. Am. 18(4), 932-939 (2001).
23. K. K. Tremper,”Pulse oximetry,” Chest 95(4), 713-715 (1989).
24. H. Duadi, M. Nitzan, and D. Fixler, “Simulation of oxygen saturation measurement in a single blood vein,” Opt. Lett. 41(18), 4312-4315 (2016).
25. M. Nitzan, S. Noach, E. Tobal, Y. Adar, Y. Miller, E. Shalom, and S. Engelberg, “Calibration-Free Pulse Oximetry Based on Two Wavelengths in the Infrared — A Preliminary Study,” Sensors 14(4), 7420-7434 (2014).
26. V. V. Tuchin, A. N. Bashkatov, E. A. Genina, V. I. Kochubey, V. V. Lychagov, S. A. Portnov, N. A. Trunina, D. R. Miller, S. Cho, H. Oh, B. Shim, M. Kim, J. Oh, H. Eum, Y. Ku, D. Kim, and Y. Yang, “Finger tissue model and blood perfused skin tissue phantom,” Proc. SPIE 7898, 78980Z (2011).
27. R. Ankri, H. Taitelbaum, and D. Fixler, “On Phantom experiments of the photon migration model in tissues,” The Open Optics Journal 5(1), 28-32 (2011). Crossref
28. J. S. Dam, C. B. Pedersen, T. Dalgaard, P. E. Fabricius, P. Aruna, and S. Andersson-Engels, “Fiber-optic probe for noninvasive real-time determination of tissue optical properties at multiple wavelengths,” Applied optics 40(7), 1155-1164 (2001). Crossref
29. I. Feder, M. Wróbel, H. Duadi, M. Jędrzejewska-Szczerska, and D. Fixler, “Experimental results of full scattering profile from finger tissue-like phantom,” Biomed. Opt. Express 7(11), 4695-4701 (2016). Crossref
© 2014-2025 Authors
Public Media Certificate (RUS). 12+