Laser modification of liquid nutrient medium and antibiotic solutions with the assessment by the bacteria growth dynamics

Alexander N. Malov
Irkutsk National Research Technical University, Russia

Anna V. Neupokoeva (Login required)
Irkutsk State Medical University, Russia

Lubov A. Kokorina
Irkutsk State Medical University, Russia


Paper #3149 received 9 Jan 2017; revised manuscript received 3 Mar 2017; accepted for publication 6 Mar 2017; published online 1 Apr 2017.

DOI: 10.18287/JBPE17.03.020303

Abstract

The processes of laser photoinduced modification of nutrient media and antibiotic solutions are considered. It is shown experimentally that the growth of colonies number is slower on the irradiated medium. The impact of laser radiation on the antibiotic activity is also studied experimentally. It is found that the laser-induced photomodification can affect the antimicrobial activity of the medicinal preparations.

Keywords

Bacteria; antibiotic; Laser activation; Culture medium

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References


1. Laser Therapy and Prophylactics, A.V. Kartelishev et al. (Eds.), Practical Medicine, Moscow (2012) [in Russian].

2. T. I. Karu, L. V. Pyatibrat, S. F. Kolyakov, and N. I. Afanasyeva, “Absorption measurements of cell monolayers relevant to mechanism of laser phototherapy: reduction or oxidation of cytochrome с oxidase under laser radiation at 632.8 nm,” Photomedicine and laser surgery 26(6), 593-599 (2008).

3. N. A. Tolstoy, and A. A. Spartakov, Electro-Optics and Magneto-Optics of Disperse Systems, Saint-Petersburg University Publishers, Saint-Petersburg (1996) [in Russian].

4. A. V. Finkelshtein, and O. B. Ptitsyn, Protein Physics, Book house “University”, Moscow (2002) [in Russian].

5. V. D. Lakhno, Clusters in Physics, Chemistry, Biology, Regular and chaotic dynamics, Izhevsk (2001) [in Russian].

6. A. N. Malov, and A. A. Vaichas, Laser Control of Biomineral Formation. Physical Principles and Prospects of Developing New Medical Nanotechnology, Palmarium Academic Publishing, Saarbrucken, Deutschland (2016). ISBN 978-3-659-60476-8 [in Russian].

7. A. N. Malov, A. Yu. Seteikin, A. V. Neupokoeva, E. S. Musatova, I. E. Golub, L. V. Sorokina,

8. V. S. Fetschenko, and A. A. Vaichas, “The laser radiation action on the biological objects,” Optik 124(23), 6034-6041 (2013).

9. R. A. Paringer, A. V. Kupriyanov, and N. Y. Ilyasova, “Dendritic crystallogram images classification,” Journal of Biomedical Photonics & Engineering, 1(2), 135-138 (2015).

10. A. N. Malov, A. V. Neupokoeva, and A. Yu. Sambyalova, “Diagnostics of liquid properties by the example of bioorganic solution,” Journal of Biomedical Photonics & Engineering 2(1), 010304 (2016).

11. A. N. Malov, A. V. Neupokoeva, E. M. Kudryashova, “Craquelure structure of protein film modification under the laser radiation of different spectral range action,” Journal of Biomedical Photonics & Engineering 2(1), 010307 (2016).

12. Determination of antimicrobial activity of antibiotics using the method of diffusion into agar. State Quality Standard of Medical Agent. General Pharmacopeia Article, (GPA replace to GP XII, part 1, GPA 42-0068-07), Ministry of Health of Russian Federation (2010) [in Russian].




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