Diagnostics of Oxidative Stress by Laser Optical-Acoustic Spectroscopy

Аndrey А. Boyko
Tomsk State University, Russia

Alexey V. Borisov
Tomsk State University, Russia

Vyacheslav S. Zasedatel
Tomsk State University, Russia

Vyacheslav V. Romanchuk
Tomsk State University, Russia

Yury V. Kistenev (Login required)
Tomsk State University, Russia

Paper #3522 received 10 Sep 2022; revised manuscript received 16 Oct 2022; accepted for publication 17 Oct 2022; published online 12 Dec 2022.

DOI: 10.18287/JBPE22.08.040301


The results of the registration of oxidative stress volatile molecular markers (NOx, C2H6, and C5H12) in exhaled air by optical-acoustic spectroscopy are presented. Aerobic physical exercises were chosen as a method of oxidative stress activation. Three time points were studied: before exercises, immediately after exercises, and after 25 min rest. It was shown that there is a statistically significant increase in the studied markers concentrations in the exhaled air immediately after the exercises. After the rest, statistically significant differences are observed in relation to the initial state only for a part of these markers. This is most likely caused by different recovery of the subjects after the exercises and insufficient rest time. Thus, this instrumental approach is promising for non-invasive registration of markers of oxidative stress.


oxidative stress; human exhalation analysis; non-invasive diagnostics; optical parametric oscillator; laser optical-acoustic spectroscopy

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1. R. Kohen, A. Nyska, “Oxidation of biological systems: Oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantication,” Toxicologic Pathology 30(6), 620–650 (2002).

2. E. D. Lephart, “Skin aging and oxidative stress: Equol’s anti-aging effects via biochemical and molecular mechanisms,” Ageing Research Reviews 31, 36–54 (2016).

3. B. Halliwell, “Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies,” Free Radical Research 29(6), 469–486 (1998).

4. W. M. Winnik, K. T. Kitchin, “Measurement of oxidative stress parameters using liquid chromatography-tandem mass spectroscopy (LC-MS/MS),” Toxicology and Applied Pharmacology 233(1), 100–106 (2008).

5. E. V. Kalinina, N. N. Chernov, and M. D. Novichkova, “Role of glutathione, glutathione transferase, and glutaredoxin in regulation of redox-dependent processes,” Biochemistry (Moscow) 79(13), 1562–83 (2014).

6. J. W. Stephens, M. P. Khanolkar, and S. C. Bain, “The biological relevance and measurement of plasma markers of oxidative stress in diabetes and cardiovascular disease,” Atherosclerosis 202(2), 321–329 (2009).

7. M. Krzystek-Korpacka, B. Salmonowicz, D. Boehm, I. Berdowska, B. Zielinski, E. Patryn, A. Noczynska, and A. Gamian, “Diagnostic potential of oxidative stress markers in children and adolescents with type 1 diabetes,” Clinical Biochemistry 41(1–2), 48–55 (2008).

8. D. Giustarinil, I. Dalle-Donne, D. Tsikas, and R. Rossil, “Oxidative stress and human diseases: Origin, link, measurement, mechanisms, and biomarkers,” Critical Reviews in Clinical Laboratory Science 46(5–6), 241–281 (2009).

9. W. Cao, Y. Duan, “Breath Analysis: Potential for Clinical Diagnosis and Exposure Assessment,” Clinical Chemistry 52(5), 800–811 (2006).

10. I. Horváth, L. E. Donnelly, A. Kiss, P. Paredi, S. A. Kharitonov, and P. J. Barnes, “Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma: a new marker of oxidative stress,” Thorax 53, 668–672 (1998).

11. S. A. Kharitonov, “Exhaled nitric oxide and carbon monoxide in respiratory diseases other than asthma,” European Respiratory Journal 9, 223–226 (1999).

12. S. A. Kharitonov, P. J. Barners, “Exhaled markers of pulmonary disease,” American Journal of Respiratory and Critical Care Medicine 163(7), 1693–1722 (2001).

13. B. Buszewski, M. Kesy, T. Ligor, and A. Amann, “Human exhaled air analytics: biomarkers of diseases,” Biomedical Chromatography 21(6), 553–566 (2007).

14. E. Casimirri, M. Stendardo, M. Bonci, R. Andreoli, B. Bottazzi, R. Leone, M. Schito, A. Vaccari, A. Papi, M. Contoli, M. Corradi, and P. Boschetto, “Biomarkers of oxidative-stress and inflammation in exhaled breath condensate from hospital cleaners,” Biomarkers 2(21), 115–122 (2015).

15. H. P. Chan, V. Tran, “Elevated levels of oxidative stress markers in exhaled breath condensate,” Journal of Thoracic Oncology 2(4), 172–178 (2009).

16. S. K. Powers, M. C. Hogan, “Exercise and oxidative stress,” Journal of Physiology 594(18), 5079–5080 (2016).

17. T. Kawamura, I. Muraoka, “Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint,” Antioxidants 7(9), 119 (2018).

18. S. Schiavone, V. Jaquet, L. Trabace, and K.-H. Krause, “Severe life stress and oxidative stress in the brain: From animal models to human pathology,” Antioxidants & Redox Signaling 18(12), 1475–1490 (2013).

19. A. Morales, A. Perez-Jimenez, A. Pérez-Jiméneza, M. C. Hidalgo, E. Abellán, and G. Cardenete, “Oxidative stress and antioxidant defenses after prolonged starvation in Dentex liver,” Comparative Biochemistry and Physiology 139(1–3), 153–161 (2004).

20. D. Wu, A. Cederbaum, “Alcohol, oxidative stress, and free radical damage,” Alcohol Research Health 27(4), 277–284 (2003).

21. K. Woods, P. Bishop, and E. Jones, “Warm-up and stretching in the prevention of muscular injury,” Sports Medicine 37(12), 1089–1099 (2007).

22. A. A. Karapuzikov, I. V. Sherstov, D. B. Kolker, A. I. Karapuzikov, Yu. V. Kistenev, D. A. Kuzmin, M. Yu. Shtyrov, N. Yu. Dukhovnikova, K. G. Zenov, A. A. Boyko, M. K. Starikova, I. I. Tikhonyuk, I. B. Miroshnichenko, M. B. Miroshnichenko, Yu. B. Myakishev, and V. N. Lokonov, “LaserBreeze gas analyzer for noninvasive diagnostics of air exhaled by patients,” Physics of Wave Phenomena 22(3), 189–196 (2014).

23. The Information System HITRAN on the Web (accessed 10 October 2022). [https://hitran.iao.ru/].

24. NIST Chemistry WebBook (accessed 10 October 2022). [https://webbook.nist.gov/chemistry/quant-ir/].

25. A. V. Borisov, A. G. Syrkina, D. A. Kuzmin, V. V. Ryabov, A. A. Boyko, O. Zaharova, V. S. Zasedatel, and Y. V. Kistenev, “Application of machine learning and laser optical-acoustic spectroscopy to study the profile of exhaled air volatile markers of acute myocardial infarction,” Journal of Breath Research 15(2), 027104 (2021).

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