Effect of Hydrated Fullerene C60 in a Wide Concentration Range on Low-Level Photon Emission from Undiluted Human Blood

Olga I. Yablonskaya (Login required)
Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow, Russia

Vladimir L. Voeikov
Lomonosov Moscow State University, Russia

Kirill N. Novikov
Lomonosov Moscow State University, Russia

Ekaterina V. Buravleva
Lomonosov Moscow State University, Russia

Nadezhda G. Berdnikova
Sechenov Moscow State Medical University, Russia


Paper #3532 received 15 Sep 2022; revised manuscript received 30 Oct 2022; accepted for publication 30 Oct 2022; published online 17 Dec 2022.

DOI: 10.18287/JBPE22.08.040504

Abstract

Hydrated fullerene C60 (HyFnC60) is a symmetrical molecule of C60 Buckminster fullerene surrounded by a water shell obtained by an original technique. It can act as an antioxidant or a pro-oxidant in various biological objects, depending on the conditions. In this study we added HyFnC60 at a range of concentrations obtained by a method of serial dilutionswith vigorous shaking at each step to whole undiluted blood of healthy donors and hospital patients with COPD and examined lucigenin-enhanced blood chemiluminescence. We have found that HyFnC60 at concentrations of
2.5 ´ 10–6 M, 2.5 ´ 10–7 M, 2.5 ´ 10–17 M, and 2.5 ´ 10–19 M increased lucigenin-dependent chemiluminescence in heathy donors’ blood while in blood of patients with COPD it had an opposite effect. This can be interpreted as in healthy donors’ blood reactive oxygen species (ROS) generation is enhanced by HyFnC60 while in patients with a chronic inflammatory disease with already increased ROS generation it is attenuated. This indicates that HyFnC60 preparations even in ultra-high dilutions may play the role of a tuner of the processes with ROS participation. Probable reasons of such action of HyFnC60 on human blood even in ultra-high dilutions are discussed.

Keywords

undiluted blood; hydrated fullerene; chemiluminescence; reactive oxygen species; photon emission

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