Calibration Problem in Laser Ektacytometry of Erythrocytes
Sergey Y. Nikitin
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M. V. Lomonosov Moscow State University, Russia
Paper #3541 received 22 Sep 2022; revised manuscript received 24 Oct 2022; accepted for publication 25 Oct 2022; published online 12 Dec 2022.
DOI: 10.18287/JBPE22.08.040503
Abstract
The problem of measuring the deformability of erythrocytes by laser diffractometry in shear flow (ektacytometry) is considered. The question of how the shape of the isointensity line in the diffraction pattern, which arises when a laser beam is scattered by an ensemble of erythrocytes, is related to the level of light intensity on this line, is analyzed. A simplified algorithm for measuring the parameters of the distribution of erythrocytes in deformability is proposed, which does not require photometry of the central part of the diffraction pattern.
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