Silver Anchored α-MnO2 Nanorods Based SERS Substrates for Salivary Thiocyanate Detection and Application in Oral Cancer Diagnosis

Navami Sunil
PSG institute of Advanced Studies, Coimbatore, India

Rajesh Unnathpadi
PSG institute of Advanced Studies, Coimbatore, India

Biji Pullithadathil (Login required)
PSG institute of Advanced Studies, Coimbatore, India

Paper #7203 received 21 Feb 2023; revised manuscript received 19 Apr 2023; accepted for publication 24 Jun 2023; published online 31 Aug 2023.


Salivary sensors are ideal non-invasive diagnostic platforms for cancer detection owing to the existence of many proteins and metabolites that can reflect individual’s health status. Elevated levels of thiocyanate ( ) in smokers’ saliva may be a potential biomarker indicating increased oral cancer risk due to the nitrosation process. Herein, we report a highly sensitive,
label-free, ultrasensitive surface-enhanced Raman spectroscopy (SERS) detection of salivary thiocyanate using silver anchored α-MnO2 nanorods based SERS substrates for the early detection of oral cancer. The structural and morphological characterization of the synthesised Ag/ α-MnO2 nanorods have been performed using XRD, FTIR, and TEM analysis. The intensity of SERS peak at around 2170 cm−1, originating from the −C≡N stretching mode, indicated the concentrations of SCN ions. The SERS spectra of  was recorded at concentrations ranging from 1 to 6 mM, which was utilised to differentiate between smokers and non-smokers and hence to predict the risk of oral cancer. 


salivary sensors; non-invasive diagnosis; oral cancer; surface enhanced Raman spectroscopy; thiocyanate

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