Dynamic Light Scattering Analysis in Biomedical Research and Applications of Nanoparticles and Polymers

Alexander Knysh
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russian Federation

Pavel Sokolov
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russian Federation

Igor Nabiev orcid (Login required)
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russian Federation
Université de Reims Champagne-Ardenne, Reims, France

Paper #8681 received 2 Mar 2023; revised manuscript received 13 Apr 2023; accepted for publication 17 Apr 2023; published online 30 May 2023.


Dynamic light scattering (DLS) is one of the most commonly used photonic methods for estimating the hydrodynamic radius, ζ-potential, polydispersity, and concentrations of nanoparticles (NPs), polymers, and cells, as well as for studying changes in these parameters upon interaction or aggregation of molecules and particles. NPs and polymers are components of numerous drugs, cosmetics, and food industry products. Hence, the monitoring of their physical, chemical, and morphological properties, often related to their functional characteristics and toxicity, are of vital importance. This review deals with the specifics of the DLS method as applied to the analysis of samples of different types and the modifications of this method depending on the characteristics of the samples. The theoretical basis of the DLS method and its applications to the study of NPs, polymers, and their interactions are presented, with the focus on biomedical applications. The last section of the review considers the advantages and limitations of DLS analysis as compared with other photonic analytical methods, as well as future trends in the development of this approach.


dynamic light scattering; nanoparticles; polymers; hybrid materials; ζ-potential; hydrodynamic diameter

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