Self-Assembled Porphyrin Nanoparticles Interaction Analysis with Albumin by Dynamic Light Scattering

Aleksey R. Krot orcid (Login required)
Lomonosov Moscow State University, Russian Federation

Aleksander I. Ladynin orcid
Lomonosov Moscow State University, Russian Federation

Irina A. Sergeeva orcid
Lomonosov Moscow State University, Russian Federation


Paper #9058 received 14 Jan 2024; revised manuscript received 22 Apr 2024; accepted for publication 1 May 2024; published online 28 May 2024.

DOI: 10.18287/JBPE24.10.020305

Abstract

We herein introduce the study of self-assembled porphyrazine nanoparticles photosensitizers in aqueous solutions with and without a polyvinylpyrrolidone carrier, respectively. For the first time, distributions of hydrodynamic radii for each of the samples separately were obtained, as well as corresponding changes in distributions for multicomponent systems in the presence of human serum albumin. For reliable interpretation of the obtained distributions, a statistical processing method adapted to biological samples was used. The study identified combinations of photosensitizer nanoparticles that could either efficiently interact with human transport proteins or, conversely, maintain their stability in their presence. Thus, the research conducted via dynamic light scattering revealed the potential for fundamentally different passive delivery methods of these nanoparticles: both with and without interaction with the main transport protein of human blood.

Keywords

dynamic light scattering; hydrodynamic radius; photodynamic therapy; self-assembled photosensitizers; porphyrazines; human serum albumin

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