Possibility of Measuring of Erythrocyte Size Distribution Parameters by Laser Diffractometry of a Blood Smear

Sergey Yu. Nikitin; Evgeniy G. Tsybrov; Maria S. Lebedeva; Andrei E. Lugovtsov; Alexander V. Priezzhev

doi:10.18287/JBPE24.10.040302

Possibility of Measuring of Erythrocyte Size Distribution Parameters by Laser Diffractometry of a Blood Smear

Sergey Yu. Nikitin
M. V. Lomonosov Moscow State University, Russian Federation

Evgeniy G. Tsybrov (Login required)
M. V. Lomonosov Moscow State University, Russian Federation

Maria S. Lebedeva
M. V. Lomonosov Moscow State University, Russian Federation

Andrei E. Lugovtsov
M. V. Lomonosov Moscow State University, Russian Federation

Alexander V. Priezzhev
M. V. Lomonosov Moscow State University, Russian Federation

Paper #9103 received 28 Apr 2024; revised manuscript received 20 May 2024; accepted for publication 20 May 2024; published online 29 Aug 2024.

DOI: 10.18287/JBPE24.10.040302

Abstract

We consider the issues of obtaining information about the distribution of red blood cells in size from the scattering pattern of a laser beam on a wet blood smear. Within this article it is assumed to calculate the first three moments of the size distribution. The analytical model of light diffraction by blood smears was developed, as well as numerical experiments for verification suggested approach were carried out. The possibility of calculating average diameter of erythrocyte, width and asymmetry of size distribution by analyzing the angular distribution of light intensity in the diffraction pattern was shown

Keywords

light scattering; laser diffractometry; data processing algorithms; red blood cells; size distribution; asymmetry

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