Silica Microspheres with Adsorbed Gold Nanostars for Single Cell SERS Detection of Formazan

Olga A. Inozemtseva (Login required)
Saratov State University, Russian Federation
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences –
a separate structural subdivision of the Federal State Budgetary Scientific Institution Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”, Russian Federation

Ekaterina S. Prikhozhdenko
Saratov State University, Russian Federation

Olga I. Gusliakova
Saratov State University, Russian Federation

Yulia A. Tyunina
Saratov State University, Russian Federation
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences –
a separate structural subdivision of the Federal State Budgetary Scientific Institution Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”, Russian Federation

Mariia S. Saveleva
Saratov State University, Russian Federation

Daniil N. Bratashov
Saratov State University, Russian Federation

Andrey M. Burov
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences –
a separate structural subdivision of the Federal State Budgetary Scientific Institution Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”, Russian Federation

Boris N. Khlebtsov
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences –
a separate structural subdivision of the Federal State Budgetary Scientific Institution Federal Research Center “Saratov Scientific Center of the Russian Academy of Sciences”, Russian Federation


Paper #9160 received 30 Aug 2024; revised manuscript received 21 Oct 2024; accepted for publication 25 Oct 2024; published online 16 Nov 2024.

DOI: 10.18287/JBPE24.10.040308

Abstract

In this study we report a rapid and sensitive surface-enhanced Raman spectroscopy (SERS)-based 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for in situ cell viability and proliferation assessment. The developed method based on silica microspheres with adsorbed gold nanostars serves as intracellular SERS platforms for formazan detection. The proposed method has several advantages over traditional MTT assays. Firstly, the formazan concentration can be determined from the SERS signal intensity without extraction and enrichment steps. Secondly, the SERS spectra of formazan can be directly obtained at the level of a single particle internalized by the cell. The SERS-MTT detection method is expected to avoid interference from background substances (such as residual MTT reagent, serum, exogenous drugs) in cell suspensions. Furthermore, the proposed approach not only simplifies the testing procedure, but it is also anticipated to provide more precise results when evaluating the effectiveness of cancer treatments compared to the conventional MTT method.

Keywords

3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); surface-enhanced Raman spectroscopy (SERS); formazan; gold nanostars

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