Photodynamic Inactivation of Pigmented and Non-Pigmented Pseudomonas Aeruginosa in Biofilms

Sergey Letuta orcid
Orenburg State University, Russian Federation

Azamat Ishemgulov orcid (Login required)
Orenburg State University, Russian Federation

Olga Davydova
Orenburg State University, Russian Federation




DOI: 10.18287/JBPE25.11.040305

Abstract

This study presents a method to improve the efficacy of photodynamic inactivation against both pigmented and non-pigmented Pseudomonas aeruginosa strains in biofilms through pre-treatment with acoustic shock waves. Biofilms were exposed to photosensitizers in solution, and shock waves were generated using laser pulses (10 ns duration, 5–10 MW/cm² power density). Pre-treatment with shock waves significantly enhanced photodynamic bacterial damage, increasing inactivation efficiency by twofold for the non-pigmented strain and sixfold for the pigmented strain. The observed improvement is likely due to shock wave-induced permeabilization of the biofilm matrix and bacterial cell membranes, facilitating greater uptake of photosensitizers and oxygen

Keywords

biofilms; pyoverdine; shock waves; photodynamic inactivation

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