Determination of Carotenoids in the Ovaries of Healthy Cats and Cats with Leiomyosarcom
Ekaterina I. Selifonova
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Saratov State University, Russian Federation
Iuliia S. Kruss
Saratov State University, Russian Federation
Alexey A. Chernov
V.I. Razumovsky Saratov State Medical University, Russian Federation
Tatyana Yu. Rusanova
Saratov State University, Russian Federation
Valery V. Tuchin
Saratov State University, Russian Federation
Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the RAS”, Saratov, Russian Federation
Tomsk State University, Russian Federation
Paper #9433 received 18 Nov 2025; revised manuscript received 1 Dec 2025; accepted for publication 1 Dec 2025; published online 7 Apr 2026.
DOI: 10.18287/JBPE26.12.020301
Abstract
The mortality rate of ovarian cancer remains the greatest among other oncological diseases of genital system worldwide. Using carotenoids as a preventive measure against ovarian cancer and a means to improve the state of genital system in general is a popular trend when determining the nutritive factors. In our study we have determined the total content of carotenoids in healthy feline ovaries and ovaries of cats diagnosed with leiomyosarcoma. We have found that total content of carotenoids varies between left and right ovaries of the same healthy cat. However, cats diagnosed with leiomyosarcoma had significantly lower carotenoid content in both left and right ovaries. As carotenoids can be used as chemotherapeutic agents, the study of their content in ovaries is a relevant problem that requires further study. Determination of the impact of carotenoids on oncological processes may find practical use in public healthcare.
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1. R. U. K, A. P. Elango, R. Subramanian, and S. Ks, “The evolving landscape of ovarian cancer: innovations in biotechnology and artificial intelligence- based screening and treatment,” Current Treatment Options in Oncology 26(7), 622–637 (2025).
2. R. Sparić, M. Andjić, I. Babović, L. Nejković, M. Mitrović, J. Štulić, M. Pupovac, and A. Tinelli, “Molecular insights in uterine leiomyosarcoma: a systematic review,” International Journal of Molecular Sciences 23(17), 9728 (2022).
3. M. Bosoteanu, M. Deacu, R. I. Voda, C. I. Orasanu, M. Aschie, S. E. Vlad, R. C. Penciu, and S. I. Chirila, “Five-year retrospective study of uterine stump and leiomyosarcoma,” Clinics and Practice 12(6), 897–907 (2022).
4. N. Arslansoy and O. Fidan, “Carotenoids and Their Antioxidant Power,” Biochemistry 60 (2024).
5. A. Nishino, T. Ichihara, T. Takaha, T. Kuriki, H. Nihei, K. Kawamoto, H. Yasui, and T. Maoka, “Accumulation of paprika carotenoids in human plasma and erythrocytes,” Journal of Oleo Science 64(10), 1135–1142 (2015).
6. R. Srivastava, “Physicochemical, antioxidant properties of carotenoids and its optoelectronic and interaction studies with chlorophyll pigments,” Scientific Reports 11(1), 18365 (2021).
7. M. K. Dehnavi, S. Ebrahimpour-Koujan, K. Lotfi, and L. Azadbakht, “The association between circulating carotenoids and risk of breast cancer: a systematic review and dose–response meta-analysis of prospective studies,” Advances in Nutrition 15(1), 100135 (2024).
8. C. Peng, O. A. Zeleznik, K. H. Shutta, B. A. Rosner, P. Kraft, C. B. Clish, M. J. Stampfer, W. C. Willett, R. M. Tamimi, and A. H. Eliassen, “A metabolomics analysis of circulating carotenoids and breast cancer risk,” Cancer Epidemiology, Biomarkers & Prevention 31(1), 85–96 (2022).
9. E. Czeczuga-Semeniuk and S. Wołczyński, “Dietary carotenoids in normal and pathological tissues of corpus uteri,” Folia Histochemica et Cytobiologica 46(3), 283–290 (2008).
10. E. Czeczuga-Semeniuk and S. Wolczynski, “Identification of carotenoids in ovarian tissue in women,” Oncology Reports 14(5), 1385-1392 (2005).
11. E. Czeczuga-Semeniuk and S. Wołczyński, “Does variability in carotenoid composition and concentration in tissues of the breast and reproductive tract in women depend on type of lesion?,” Advances in Medical Sciences 53(2), 270-277 (2008).
12. M. Zhang, C. D. J. Holman, and C. W. Binns, “Intake of specific carotenoids and the risk of epithelial ovarian cancer,” British Journal of Nutrition 98(1), 187-193 (2007).
13. A. Koushik, D. J. Hunter, D. Spiegelman, K. E. Anderson, J. E. Buring, J. L. Freudenheim, R. A. Goldbohm, S. E. Hankinson, S. C. Larsson, M. Leitzmann, J. R. Marshall, M. L. McCullough, A. B. Miller, C. Rodriguez, T. E. Rohan, J. A. Ross, A. Schatzkin, L. J. Schouten, W. C. Willett, A. Wolk, S. M. Zhang, and S. A. Smith-Warner, “Intake of the major carotenoids and the risk of epithelial ovarian cancer in a pooled analysis of 10 cohort studies,” International Journal of Cancer 119(9), 2148–2154 (2006).
14. V. Böhm, G. Lietz, B. Olmedilla-Alonso, D. Phelan, E. Reboul, D. Bánati, P. Borel, J. Corte-Real, A. R. De Lera, C. Desmarchelier, J. Dulinska-Litewka, J.-F. Landrier, I. Milisav, J. Nolan, M. Porrini, P. Riso, J. M. Roob, E. Valanou, A. Wawrzyniak, B. M. Winklhofer-Roob, R. Rühl, and T. Bohn, “From carotenoid intake to carotenoid blood and tissue concentrations – implications for dietary intake recommendations,” Nutrition Reviews 79(5), 544–573 (2021).
15. B. P. Chew, B. B. C. Weng, H. W. Kim, T. S. Wong, J. S. Park, and A. J. Lepine, “Uptake of β-carotene by ovarian and uterine tissues and effects on steroidogenesis during the estrous cycle in cats,” American Journal of Veterinary Research 62(7), 1063–1067 (2001).
16. I. Gunyeli, M. Saygin, and O. Ozmen, “Methotrexate-induced toxic effects and the ameliorating effects of astaxanthin on genitourinary tissues in a female rat model,” Archives of Gynecology and Obstetrics 304(4), 985–997 (2021).
17. C.-S. Lei, Y.-C. Hou, M.-H. Pai, M.-T. Lin, and S.-L. Yeh, “Effects of quercetin combined with anticancer drugs on metastasis-associated factors of gastric cancer cells: in vitro and in vivo studies,” The Journal of Nutritional Biochemistry 51, 105–113 (2018).
18. N. I. Krinsky and E. J. Johnson, “Carotenoid actions and their relation to health and disease,” Molecular Aspects of Medicine 26(6), 459–516 (2005).
19. J. Shin, M.-H. Song, J.-W. Oh, Y.-S. Keum, and R. K. Saini, “Pro-oxidant actions of carotenoids in triggering apoptosis of cancer cells: a review of emerging evidence,” Antioxidants 9(6), 532 (2020).
20. C. Allemani, T. Matsuda, V. Di Carlo, R. Harewood, M. Matz, M. Nikšić, A. Bonaventure, M. Valkov, C. J. Johnson, J. Estève, O. J. Ogunbiyi, G. A. E Silva, W.-Q. Chen, S. Eser, G. Engholm, C. A. Stiller, A. Monnereau, R. R. Woods, O. Visser, G. H. Lim, J. Aitken, H. K. Weir, M. P. Coleman, CONCORD Working Group, “Global surveillance of trends in cancer survival 2000–14 (CONCORD-3): analysis of individual records for 37 513 025 patients diagnosed with one of 18 cancers from 322 population-based registries in 71 countries,” The Lancet 391(10125), 1023–1075 (2018).
21. G. L. Russo, S. Moccia, M. Russo, and C. Spagnuolo, “Redox regulation by carotenoids: Evidence and conflicts for their application in cancer,” Biochemical Pharmacology 194, 114838 (2021).
22. C. Liu, R. M. Russell, and X.-D. Wang, “Lycopene supplementation prevents smoke-induced changes in p53, p53 phosphorylation, cell proliferation, and apoptosis in the gastric mucosa of ferrets,” The Journal of Nutrition 136(1), 106–111 (2006).
23. A. A. Selifonov and V. V. Tuchin, “Optical properties of porcine oral mucosa at application of iodine preparation based on glycerol,” Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases, 116260Х (2021).
24. V. V. Tuchin, D. Zhu, and E. A. Genina, Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging, 1st ed. (CRC Press, 2021).
25. N. N. Karkischenko, V. N. Karkischenko, Yu. V. Fokin, L. A. Taboyakova, O. V. Alimkina, and M. M. Borisova, “Between cognitivity and neuropathies: neuroimaging of the effects of gabaergic modulation of the hippocampus and prefrontal neocortexis by normalized brain electrograms,” Biomedicine (2), 12–38 (2020).
26. D.B. Rodriguez-Amaya, A guide to carotenoid analysis in foods. Printed in the United States of America, ILSI Press, 64 p. (2001). ISBN: 1578810728.
27. I. V. Ermakov and W. Gellermann, “Dermal carotenoid measurements via pressure mediated reflection spectroscopy,” Journal of Biophotonics 5(7), 559–570 (2012).
28. S. Andree, C. Reble, and J. Helfmann, “Spectral in vivo signature of carotenoids in visible light diffuse reflectance from skin in comparison to ex vivo absorption spectra,” Photonics & Lasers in Medicine 2(4), (2013).
29. M. E. Darvin, C. Sandhagen, W. Koecher, W. Sterry, J. Lademann, and M. C. Meinke, “Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy,” Journal of Biophotonics 5(7), 550–558 (2012).
30. S. Masoumi, M. A. Ansari, E. Mohajerani, E. A. Genina, and V. V. Tuchin, “Combination of analytical and experimental optical clearing of rodent specimen for detecting beta-carotene: phantom study,” Journal of Biomedical Optics 23(09), 1 (2018).
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