Alternating Magnetic Field Effect on Oxygen Transport Function of Red Blood Cells in Vitro

Olga V. Slatinskaia (Login required)
M.V. Lomonosov Moscow State University, Russian Federation

Alexander V. Priezzhev
M.V. Lomonosov Moscow State University, Russian Federation

Lizaveta V. Uzlova
Grodno State Medical University, Republic of Belarus

Victor V. Zinchuk
Grodno State Medical University, Republic of Belarus

Andrei E. Lugovtsov
M.V. Lomonosov Moscow State University, Russian Federation


Paper #9544 received 1 Mar 2026; revised manuscript received 24 May 2026; accepted for publication 26 May 2026; published online 15 Jun 2026

DOI: 10.18287/JBPE26.12.020308

Abstract

The study presents data on the influence of alternating magnetic field (AMF) on blood in vitro, manifested by a decrease in hemoglobin’s affinity for oxygen and an increase in nitrate/nitrite content. The effect of short-term exposure (30 min) to AMF with the strength of 300 mT and frequency of 20 Hz (corresponding to the radiation from static magnetic field sources) on the oxygen transport function (OTF) of erythrocytes and conformation of Fe-containing hemoporphyrin and globin of hemoglobin was demonstrated using Raman spectroscopy. Alteration in the content of hemoglobin in the T and R-forms was revealed, indicating a different affinity of hemoglobin for ligands and associated with changes in the polarity of the globin amino acids. It was also demonstrated that exposure of erythrocytes to AMF for 360 s modifies their gasotransmitter-forming function. These changes can significantly influence the alteration of blood OTF and change the blood flow in meeting tissue oxygen demands. The obtained results indicate the point to the involvement of the gasotransmitter-forming function in modifying blood OTF and, consequently, in altering the adequacy of blood flow in meeting tissue oxygen demands
 

Keywords

oxygen transport function; magnetic field; erythrocyte; Raman spectroscopy

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References


1. V. V. Zinchuk, N. V. Hlutkina, “Hemoglobin affinity to oxygen during coronavirus infection: New faces of a known problem,” Journal of Evolutionary Biochemistry and Physiology 59(6), 2242–2257 (2023).

2. A.V. Muravyov, I. A. Tikhomirova, S. V. Bulaeva, V. A. Vdovin, and A. A. Muravyov, “The study of the role of individual blood characteristics in its fluidity alteration and transport efficiency,” Russian Journal of Biomechanics 16(3), 32–41 (2012). [in Russian]

3. J. L. Hoard, “Stereochemistry of Hemes and Other Metalloporphyrins: The remarkably varied stereochemistry of the iron porphyrins is utilized in the hemoprotein function,” Science 174(4016), 1295–1302 (1971).

4. Y. Sugita, M. Nagai, and Y. Yoneyama, “Circular dichroism of hemoglobin in relation to the structure surrounding the heme,” Journal of Biological Chemistry 246(2), 383–388 (1971).

5. J. Monod, J. Wyman, and J.-P. Changeux, “On the nature of allosteric transitions: A plausible model,” Journal of Molecular Biology 12(1), 88–118 (1965).

6. S. C. Goheen, L. J. Lis, O. Kucuk, M. P. Westerman, and J. W. Kauffman, “Study of intact and modified erythrocyte membranes with laser Raman spectroscopy,” Journal of Raman Spectroscopy 24(9), 599–602 (1993).

7. B. R. Wood, B. Tait, and D. McNaughton, “Micro-Raman characterisation of the R to T state transition of haemoglobin within a single living erythrocyte,” Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1539(1–2), 58–70 (2001).

8. B. R. Wood, D. McNaughton, “Micro-Raman characterization of high- and low-spin heme moieties within single living erythrocytes,” Biopolymers 67(4–5), 259–262 (2002).

9. E. S. Allakhverdiev, E. A. Rezukhina, O. V. Slatinskaya, O. V. Rodnenkov, G. V. Maksimov, and T. V. Martynyuk, “Possibility of evaluation of oxygen transport function in PAH patients on effective selexipag-based therapy,” Eurasian Heart Journal (3), 44–52 (2023). [in Russian]

10. A. N. Shalygin, S. B. Norina, and E. I. Kondorsky, “Behaviour of erythrocytes in high gradient magnetic field,” Journal of Magnetism and Magnetic Materials 31–34, 555–556 (1983).

11. R. Tao, K. Huang, “Reducing blood viscosity with magnetic fields,” Physical Review E 84(1), 011905 (2011).

12. T. E. Shalaby, M. M. Shawki, “Biophysical and biochemical changes in the characteristics of rat blood exposed to combined alternating and static magnetic fields,” Romanian Journal of Biophysics 16(3), 169–180 (2006).

13. S. Sharma, U. Singh, and V. K. Katiyar, “Magnetic field effect on flow parameters of blood along with magnetic particles in a cylindrical tube,” Journal of Magnetism and Magnetic Materials 377, 395–401 (2015).

14. E. Calabro, S. Magazu, “Unfolding-induced in hemoglobin by exposure to electromagnetic fields: A FTIR spectroscopy study,” Oriental Journal of Chemistry 30(1), 31–35 (2014).

15. V. V. Zinchuk, V. O. Lepeev, and I. E. Hulyai, “Participation of Gas Transmitters in the Modification of Blood Oxygen Transport Function under the Effect of a Magnetic Field,” I.M. Sechenov Russian Physiological Journal 102(10), 1176–1184 (2016).

16. V. V. Zinchuk, V. O. Lepeev, “The in vitro Effect of a Magnetic Field on the Oxygen-Transport Function and the Gaseous Transmitter System in Blood,” Biophysics 63(3), 436–440 (2018).

17. J. W. Severinghaus, “Blood gas calculator,” Journal of Applied Physiology 21(3), 1108–1116 (1966).

18. I. Guevara, J. Iwanejko, A. Dembińska-Kieć, J. Pankiewicz, A. Wanat, P. Anna, I. Gołąbek, S. Bartuś, M. Malczewska-Malec, and A. Szczudlik, “Determination of nitrite/nitrate in human biological material by the simple Griess reaction,” Clinica Chimica Acta 274(2), 177–188 (1998).

19. N. A. Brazhe, S. Abdali, A. R. Brazhe, O. G. Luneva, N. Y. Bryzgalova, E. Y. Parshina, O. V. Sosnovtseva, and G. V. Maksimov, “New insight into erythrocyte through in vivo surface-enhanced Raman spectroscopy,” Biophysical Journal 97(12), 3206–3214 (2009).

20. B. R. Wood, L. Hammer, and D. McNaughton, “Resonance Raman spectroscopy provides evidence of heme ordering within the functional erythrocyte,” Vibrational Spectroscopy 38(1–2), 71–78 (2005).

21. K. R. Ward, I. T. Filho, R. W. Barbee, L. Torres, M. H. Tiba, P. S. Reynolds, R. N. Pittman, R. R. Ivatury, and J. Terner, “Resonance Raman spectroscopy: A new technology for tissue oxygenation monitoring,” Critical Care Medicine 34(3), 792–799 (2006).

22. C. Layssol-Lamour, T. Lavabre, J. Braun, C. Trumel, and N. Bourgès-Abella, “The effects of storage at 4°C and 20°C on the hemograms of C57BL/6 mice and Wistar rats using the IDEXX ProCyte Dx and blood smear evaluations”, Veterinary Clinical Pathology 48(4), 652–667 (2019).

23. G. A. Zalesskaya, E. G. Sambor, and A. V. Kuchinskii, “Effect of intravenous laser irradiation on the molecular structure of blood and blood components,” Journal of Applied Spectroscopy 73(1), 115–122 (2006).

24. O. V. Kosmachevskaya, E. I. Nasybullina, V. N. Blindar, and A. F. Topunov, “Binding of erythrocyte hemoglobin to the membrane to realize signal-regulatory function,” Applied Biochemistry and Microbiology 55(2), 83–98 (2019).

25. M. Meuwly, M. Karplus, “The functional role of the hemoglobin-water interface,” Molecular Aspects of Medicine 84, 101042 (2022).

26. S. Mayda, Z. Kandemir, N. Bulut, and S. Maekawa, “Magnetic mechanism for the biological functioning of hemoglobin,” Scientific Reports 10(1), 8569 (2020).




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