Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero

Narendran Sudheendran
Department of Biomedical Engineering, University of Houston, TX, USA

Maleeha Mashiatulla
Department of Biomedical Engineering, University of Houston, TX, USA

Raksha Raghunathan
Department of Biomedical Engineering, University of Houston, TX, USA

Saba H. Syed
Department Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA

Mary E. Dickinson
Department Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA

Irina V. Larina
Department Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA

Kirill V. Larin (Login required)
Department of Biomedical Engineering, University of Houston, TX, USA
Department Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA
Institute of Optics and Biophotonics, Saratov State University, Russia


Paper #2039 received 2015.01.13; revised manuscript received 2015.02.25; accepted for publication 2015.02.26; published online 2015.03.28.

DOI: 10.18287/jbpe-2015-1-1-90

Abstract

Mouse models are commonly used as research tools to understand regulatory pathways affected by human diseases and disorders. Live imaging tools for visualization of mouse embryonic ocular tissues would be beneficial in research associated with developmental ocular defects. In this study, in utero quantitative assessment of ocular structures in mouse embryos was performed with a swept-source optical coherence tomography (SSOCT). To define developmental changes in eye morphology in live embryos, the volume of the embryonic eye lens and the globe at different embryonic stages ranging from E13.5 to E18.5 was quantified. It is determined that the major axis diameter of the eye lens and the globe was found to increase from 0.44±0.18 mm to 0.98±0.05 mm and from 0.56±0.22 mm to 1.23±0.14 mm, respectively, as the embryo ages from E13.5 to E18.5. For the same stages, the volume of the eye lens and globe was found to increase from 0.028±0.027 mm3 to 0.32±0.08 mm3 and from 0.085±0.08 mm3 to 0.75±0.27 mm3, respectively. These results suggest that OCT can accurately assess developmental processes of ocular structures and can be potentially used to assess embryonic ocular growth in mouse mutants with eye abnormalities and to study the effect of toxicological and pharmacological agents.

Keywords

Eye volume; in utero; mouse embryo; optical coherence tomography

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References


1. J. V. Gross, and B. D. Perkins, “Zebrafish mutants as models for congenital ocular disorders in humans,” Mol Reprod Dev 75, 547-555 (2008). Crossref

2. N. Goodall, et al., “3-Dimensional modelling of chick embryo eye development and growth using high resolution magnetic resonance imaging,” Exp Eye Res 89, 511-521 (2009). Crossref

3. Y. Ai, et al., “A mouse model of galactose-induced cataracts,” Hum Mol Genet 9, 1821-1827 (2000). Crossref

4. M. G. Anderson, et al., “Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice,” Nat Genet, 30, 81-85 (2002). Crossref

5. J. J. Windle, et al., “Retinoblastoma in transgenic mice,” Nature 343, 665-669 (1990). Crossref

6. N. A. Syed, et al., “Transgenic mice with pigmented intraocular tumors: tissue of origin and treatment,” Invest Ophthalmol Vis Sci. 39, 2800-2805 (1998).

7. P. Parasoglou, et al., “High-resolution MRI of early-stage mouse embryos,” NMR Biomed, (2012). Crossref

8. S. W. John, et al., “Essential iris atrophy, pigment dispersion, and glaucoma in DBA/2J mice,” Invest Ophthalmol Vis Sci 39, 951-962 (1998).

9. F. S. Foster, et al., “In vivo imaging of embryonic development in the mouse eye by ultrasound biomicroscopy,” Investigative ophthalmology & visual science 44, 2361-2366 (2003). Crossref

10. A. S. Brown, et al., “Quantitation of hemodynamic function during developmental vascular regression in the mouse eye,” Investigative ophthalmology & visual science 46, 2231-7 (2005). Crossref

11. D. Huang, et al., “Optical coherence tomography,” Science 254, 1178-1181 (1991).

12. I. V. Larina, et al., “Live imaging of blood flow in mammalian embryos using Doppler swept-source optical coherence tomography,” J Biomed Opt. 13, 060506 (2008). Crossref

13. I. V. Larina, et al., “Hemodynamic measurements from individual blood cells in early mammalian embryos with Doppler swept source OCT,” Opt. Lett. 34, 986-988 (2009). Crossref

14. N. Sudheendran, et al., “Speckle variance OCT imaging of the vasculature in live mammalian embryos,” Laser Phys Lett. 8, 247-252 (2011). Crossref

15. I. V. Larina, et al., “Sequential Turning Acquisition and Reconstruction (STAR) method for four-dimensional imaging of cyclically moving structures,” Biomed Opt Express 3, 650-660 (2012). Crossref

16. S. H. Syed, et al., “Optical coherence tomography for high-resolution imaging of mouse development in utero,” Journal of biomedical optics 16, 046004 (2011). Crossref

17. I. V. Larina, et al., “Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models,” Journal of biomedical optics 17, 081410 (2012). Crossref

18. V. Tuchin, Tissue Optics - Light Scattering Methods and Instruments for Medical Diagnosis, 2nd Edition, SPIE Press, Bellingham, WA (2007).

19. T. Ramaesh, et al., “Corneal abnormalities in Pax6(+/-) small eye mice mimic human aniridia-related keratopathy,” Investigative ophthalmology & visual science 44, 1871-1878 (2003). Crossref

20. E. Steingrimsson, et al., “Molecular-Basis of Mouse Microphthalmia (Mi) Mutations Helps Explain Their Developmental and Phenotypic Consequences,” Nat Genet 8, 256-263 (1994). Crossref

21. C. Harch, H. B. Chase and N. I. Gonsalves, “Studies on an Anophthalmic Strain of Mice .6. Lens and Cup Interaction,” Dev Biol. 63, 352-357 (1978).




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