Analysis of albumin Raman scattering registration efficiency from different volume and shape cuvette

Anastasia A. Lykina; Dmitry N. Artemyev; Ivan A. Bratchenko

doi:10.18287/JBPE17.03.020309

Authors

Anastasia A. Lykina Samara National Research University
Dmitry N. Artemyev Samara National Research University, Russia
Ivan A. Bratchenko Samara National Research University, Russia

DOI:

https://doi.org/10.18287/JBPE17.03.020309

Keywords:

albumin, raman spectroscopy, PLS

Abstract

The cuvette shape influence on the Raman registration efficiency at small volumes of albumin (0.15-1 ml) using the PLS model was analysed. This paper examines the smallest measurement error RMSE of 3-6 g/l with a correlation coefficient R² = 0.95 was achieved for pure Raman spectra extraction by baseline correction with asymmetric least squares smoothing method for aluminum spherical type cuvette-reflectors. Its curvature center was matched with the focal spot of the exciting laser. The error of albumin concentration determination equaled about 5% of albumin normal concentration in a blood plasma using a spherical cuvette with a volume of 0.55 ml. A further decrease of the cuvette volume led to a sharp loss of the method prediction accuracy.

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