Analysis of albumin Raman scattering registration efficiency from different volume and shape cuvette

Anastasia A. Lykina (Login required)
Samara National Research University, Russia

Dmitry N. Artemyev
Samara National Research University, Russia

Ivan A. Bratchenko
Samara National Research University, Russia

Paper #3157 received 7 Feb 2017; revised manuscript received 16 Apr 2017; accepted for publication 7 May 2017; published online 15 May 2017.

DOI: 10.18287/JBPE17.03.020309


The cuvette shape influence on the Raman registration efficiency at small volumes of albumin (0.15-1 ml) using the PLS model was analysed. This paper examines the smallest measurement error RMSE of 3-6 g/l with a correlation coefficient R2 = 0.95 was achieved for pure Raman spectra extraction by baseline correction with asymmetric least squares smoothing method for aluminum spherical type cuvette-reflectors. Its curvature center was matched with the focal spot of the exciting laser. The error of albumin concentration determination equaled about 5% of albumin normal concentration in a blood plasma using a spherical cuvette with a volume of 0.55 ml. A further decrease of the cuvette volume led to a sharp loss of the method prediction accuracy.


albumin; raman spectroscopy; PLS

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1. B. Blomback, and L. A. Hanson, Plasma proteins, John Willey & Sons (1979). ISBN: 978-0-471-99730-6.

2. C. A. Burtis, E. R. Ashwood, and D. E. Bruns, Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th ed., Elsevier Saunders, Philadelphia (2006). ISBN: 978-0-7216-0189-2.

3. F. D. Keren, Protein Electrophoresis in Clinical Diagnosis, Hodder Arnold, London (2003). ISBN: 978-0-340-81213-6. Crossref

4. M. Jermyn, J. Desroches, K. Aubertin, K. St-Arnaud, W.-J. Madore, E. De Montigny, M.-C. Guiot, D. Trudel, B. C. Wilson, and K. Petrecca, “A review of Raman spectroscopy advances with an emphasis on clinical translation challenges in oncology,” Phys. Med. Biol. 61(23), R370-R400 (2016).

5. N. C. Dingari, G. L. Horowitz, J. W. Kang, R. R. Dasari, and I. Barman, “Raman Spectroscopy Provides a Powerful Diagnostic Tool for Accurate Determination of Albumin Glycation,” PLoS ONE 7(2), 1-11(2012).

6. A. J. Berger, T.-W. Koo, I. Itzkan, G. Horowitz, and M. S. Feld, “Multicomponent blood analysis by near-infrared Raman spectroscopy,” Appl Opt. 38(13), 2916-2926 (1999). Crossref

7. A. M. K. Enejder, T.-W. Koo, J. Oh, M. Hunter, S. Sasic, M. S. Feld, and G. L. Horowitz, “Blood analysis by Raman spectroscopy,” Optics letters 27(22), 2004-2006 (2002). Crossref

8. A. Bonifacio, S. Dalla Marta, R. Spizzo, S. Cervo, A. Steffan, A. Colombatti, and V. Sergo, “Surface- enhanced Raman spectroscopy of blood plasma and serum using Ag and Au nanoparticles: A systematic study,” Analytical and Bioanalytical Chemistry 406(9), 3271-3275 (2015).

9. Z. O. Kralova, A. Orinak, R. Orinakova, L. Skantarova, and J. Radonak, “Enhanced Detection of Human Plasma Proteins on Nanostructured Silver Surfaces,” Nanomater. Nanotechnol. 3(17), 1-8 (2014).

10. D. N. Artemyev, V. P. Zakharov, I. L. Davydkin, J. A. Khristoforova, A. A. Lykina, V. N. Konyukhov, and T. P. Kuzmina, “Measurement of human serum albumin concentration using Raman spectroscopy setup,” Opt Quant Electron 48(6), 337 (2016). Crossref

11. К. Esbensen, Multivariate Data Analysis – In Practice, 4th ed., CAMO, NJ (2000).

12. S. Wold, M. Sjöströma, and L. Erikssonb, “PLS-regression: a basic tool of chemometrics,” Chemometrics and Intelligent Laboratory Systems 58(2), 109-130 (2001). Crossref

13. R. Bro, and A. K. Smilde, “Centering and scaling in component analysis,” J. Chemom. 17(1), 16-33 (2003). Crossref

14. Ch. Ruffin, and R. L. King, “The analysis of hyperspectral data using Savitzky-Golay filtering-theoretical basis,” IEEE International 2, 756-758 (1999).

15. S.-J. Baek, A. Park, Y.-J. Ahn, J. Choo, “Baseline correction using asymmetrically reweighted penalized least squares smoothing,” The Analyst 140(1), 250-257 (2015).

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