Hyperspectral Holography and Laser Diffractometry of Erythrocytes

Andrei E. Lugovtsov (Login required)
Department of Physics, M.V. Lomonosov Moscow State University, Russia
International Laser Center, M.V. Lomonosov Moscow State University, Russia

Vladislav D. Ustinov
Moscow Center of Fundamental and Applied Mathematics, M.V. Lomonosov Moscow State University, Russia

Georgy S. Kalenkov
Institute of Geosphere Dynamics (IDG RAS), Moscow, Russia

Sergey G. Kalenkov
Moscow Polytechnic University, Moscow, Russia

Alexander V. Priezzhev
Department of Physics, M.V. Lomonosov Moscow State University, Russia
International Laser Center, M.V. Lomonosov Moscow State University, Russia

Paper #3368 received 22 May 2020; revised manuscript received 18 Jun 2020; accepted for publication 18 Jun 2020; published online 24 Jun 2020.

DOI: 10.18287/JBPE20.06.020306


Geometrical 3D models of human red blood cells in dry smear were obtained experimentally using hyperspectral holography technique. Corresponding diffraction patterns in the far field diffraction zone were calculated. Visibility values of the diffraction patterns were obtained in cases of low and high red cell distributions width (RDW). Our study reveals that although the visibility is influenced by the cells shapes, this parameter can still be used to assess red cell distribution width in clinical practice.


laser diffractometry; hyperspectral holography; erythrocyte; visibility

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